Repair of full-thickness cartilage defects using liposomal transforming growth factor-β1

Tomoyuki Abe, Harumoto Yamada, Hideto Nakajima, Toshiyuki Kikuchi, Hironari Takaishi, Takushi Tadakuma, Kyosuke Fujikawa, Yoshiaki Toyama

Research output: Contribution to journalArticle

21 Citations (Scopus)

Abstract

Transforming growth factor-β1 (TGFβ1) is a well-known, potent growth factor implicated in both in vitro and in vivo chondrogenesis. Liposomes have been employed as a drug delivery system to promote the efficient use of drugs. The objective of this study was to demonstrate that a single injection of liposomal TGFβ1 has an accelerating effect on the repair of an articular cartilage defect. Full-thickness articular cartilage defects were prepared on the patellar grooves of the femurs in knee joints of Japanese white rabbits. One week after surgery, various reagents including liposomal TGFβ1, free TGFβ1, and phosphate-buffered saline were injected into the operated knee joints. At 3 weeks after surgery the specimens obtained from the lesions were evaluated histologically, and the glycosaminoglycan content was quantified. Histological examination revealed that the defects were filled with thicker fibrous cartilage and showed more intense metachromatic staining in the liposomal TGFβ1 group than in the other groups. The glycosaminoglycan content of the repair tissue was also significantly higher in the liposomal TGFβ1 group than in the other groups. This study indicated that the intraarticular injection of liposomal TGFβ1 could accelerate the early-stage repair of full-thickness articular cartilage defects.

Original languageEnglish
Pages (from-to)92-101
Number of pages10
JournalJournal of Orthopaedic Science
Volume8
Issue number1
DOIs
Publication statusPublished - 01-01-2003

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Transforming Growth Factors
Cartilage
Articular Cartilage
Knee Joint
Glycosaminoglycans
Chondrogenesis
Intra-Articular Injections
Drug Delivery Systems
Liposomes
Femur
Intercellular Signaling Peptides and Proteins
Phosphates
Staining and Labeling
Rabbits
Injections
Pharmaceutical Preparations

All Science Journal Classification (ASJC) codes

  • Orthopedics and Sports Medicine

Cite this

Abe, Tomoyuki ; Yamada, Harumoto ; Nakajima, Hideto ; Kikuchi, Toshiyuki ; Takaishi, Hironari ; Tadakuma, Takushi ; Fujikawa, Kyosuke ; Toyama, Yoshiaki. / Repair of full-thickness cartilage defects using liposomal transforming growth factor-β1. In: Journal of Orthopaedic Science. 2003 ; Vol. 8, No. 1. pp. 92-101.
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Abe, T, Yamada, H, Nakajima, H, Kikuchi, T, Takaishi, H, Tadakuma, T, Fujikawa, K & Toyama, Y 2003, 'Repair of full-thickness cartilage defects using liposomal transforming growth factor-β1', Journal of Orthopaedic Science, vol. 8, no. 1, pp. 92-101. https://doi.org/10.1007/s007760300016

Repair of full-thickness cartilage defects using liposomal transforming growth factor-β1. / Abe, Tomoyuki; Yamada, Harumoto; Nakajima, Hideto; Kikuchi, Toshiyuki; Takaishi, Hironari; Tadakuma, Takushi; Fujikawa, Kyosuke; Toyama, Yoshiaki.

In: Journal of Orthopaedic Science, Vol. 8, No. 1, 01.01.2003, p. 92-101.

Research output: Contribution to journalArticle

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