TY - JOUR
T1 - Repeated activation of Trpv1-positive sensory neurons facilitates tumor growth associated with changes in tumor-infiltrating immune cells
AU - Tanaka, Kenichi
AU - Kondo, Takashige
AU - Narita, Michiko
AU - Muta, Takeru
AU - Yoshida, Sara
AU - Sato, Daisuke
AU - Suda, Yukari
AU - Hamada, Yusuke
AU - Tezuka, Hiroyuki
AU - Kuzumaki, Naoko
AU - Narita, Minoru
N1 - Funding Information:
This research was supported by the National Cancer Center Research and Development Fund ( 2020-A-1 ) and Hoshi University . We thank Y Ide, M Iwabuchi, R Kakutani, R Tatezuki, T Hojo and H Mochizuki for their help with the experiments.
Publisher Copyright:
© 2023
PY - 2023/3/12
Y1 - 2023/3/12
N2 - It is considered that sensory neurons extend into the tumor microenvironment (TME), which could be associated with tumor growth. However, little is known about how sensory signaling could promote tumor progression. In this study, chemogenetic activation of transient receptor potential vanilloid 1 (Trpv1)-positive sensory neurons (C-fibers) by the microinjection of AAV-hSyn-FLEX-hM3Dq-mCherry into the sciatic nerve dramatically increased tumor volume in tumor-bearing Trpv1-Cre mice. This activation in Trpv1::hM3Dq mice that had undergone tumor transplantation significantly reduced the population of tumor-infiltrating CD4+ T cells and increased the mRNA level of the M2-macrophage marker, CX3C motif chemokine receptor 1 (Cx3cr1) in immunosuppressive cells, such as tumor-associated macrophages (TAMs) and tumor-infiltrating monocytic myeloid-derived suppressor cells (M-MDSCs). Under these conditions, we found a significant correlation between the decreased expression of the M1-macrophage marker Tnf and tumor volume. These findings suggest that repeated activation of Trpv1-positive sensory neurons may facilitate tumor growth along with changes in tumor-infiltrating immune cells.
AB - It is considered that sensory neurons extend into the tumor microenvironment (TME), which could be associated with tumor growth. However, little is known about how sensory signaling could promote tumor progression. In this study, chemogenetic activation of transient receptor potential vanilloid 1 (Trpv1)-positive sensory neurons (C-fibers) by the microinjection of AAV-hSyn-FLEX-hM3Dq-mCherry into the sciatic nerve dramatically increased tumor volume in tumor-bearing Trpv1-Cre mice. This activation in Trpv1::hM3Dq mice that had undergone tumor transplantation significantly reduced the population of tumor-infiltrating CD4+ T cells and increased the mRNA level of the M2-macrophage marker, CX3C motif chemokine receptor 1 (Cx3cr1) in immunosuppressive cells, such as tumor-associated macrophages (TAMs) and tumor-infiltrating monocytic myeloid-derived suppressor cells (M-MDSCs). Under these conditions, we found a significant correlation between the decreased expression of the M1-macrophage marker Tnf and tumor volume. These findings suggest that repeated activation of Trpv1-positive sensory neurons may facilitate tumor growth along with changes in tumor-infiltrating immune cells.
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U2 - 10.1016/j.bbrc.2023.01.075
DO - 10.1016/j.bbrc.2023.01.075
M3 - Article
C2 - 36724558
AN - SCOPUS:85147434080
SN - 0006-291X
VL - 648
SP - 36
EP - 43
JO - Biochemical and Biophysical Research Communications
JF - Biochemical and Biophysical Research Communications
ER -