Repeated glucoprivation delayed hyperphagic responses while activating neuropeptide Y neurons in rats

Yoshiharu Ozawa, Hiroshi Arima, Minemori Watanabe, Hiroshi Shimizu, Yoshihiro Ito, Ryoichi Banno, Yoshihisa Sugimura, Nobuaki Ozaki, Hiroshi Nagasaki, Yutaka Oiso

Research output: Contribution to journalArticle

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Abstract

It is well known that glucoprivation induces the release of counterregulatory hormones such as glucagon, and that the response is attenuated when the stimuli are repeated. Glucoprivation also activates orexigenic neurons and induces hyperphagic responses, although it remains unclear whether these responses are attenuated in repeated glucoprivation. In this study, we examined time course changes in feeding as well as activities of orexigenic neuropeptide Y (NPY) neurons in repeated glucoprivation in rats. Either 2-deoxy-d-glucose (2DG), which blocks glucose utilization, or isotonic saline (control) was injected subcutaneously to rats for 14 days, and food consumption for 1 and 2 h after injection was monitored throughout the experiment. While 2DG injection induced robust feeding responses during the first 1 h after injection, the response was gradually attenuated and the food consumption was significantly less on days 12-14 compared to that on day 1. On the other hand, food consumption during 2 h after 2DG injection was not changed significantly for 14 days. The transcriptional activities of NPY neurons in the arcuate nucleus and C1/A1 region of the hindbrain, measured by intronic in situ hybridization, were significantly enhanced after repeated 2DG injection for 14 days, while the feeding responses to intracerebroventricular injection of NPY were significantly less in the 2DG-repeated group compared to the saline-repeated group. It is thus demonstrated that repeated glucoprivation delayed hyperphagic responses while activating NPY neurons in rats. Our data also suggest that decreased feeding responses to NPY might be at least partially responsible for the delayed response.

Original languageEnglish
Pages (from-to)763-769
Number of pages7
JournalPeptides
Volume32
Issue number4
DOIs
Publication statusPublished - 01-04-2011

Fingerprint

Neuropeptide Y
Neurons
Rats
Glucose
Injections
Food
Rhombencephalon
Arcuate Nucleus of Hypothalamus
Glucagon
In Situ Hybridization
Hormones
Experiments

All Science Journal Classification (ASJC) codes

  • Biochemistry
  • Physiology
  • Endocrinology
  • Cellular and Molecular Neuroscience

Cite this

Ozawa, Y., Arima, H., Watanabe, M., Shimizu, H., Ito, Y., Banno, R., ... Oiso, Y. (2011). Repeated glucoprivation delayed hyperphagic responses while activating neuropeptide Y neurons in rats. Peptides, 32(4), 763-769. https://doi.org/10.1016/j.peptides.2010.12.009
Ozawa, Yoshiharu ; Arima, Hiroshi ; Watanabe, Minemori ; Shimizu, Hiroshi ; Ito, Yoshihiro ; Banno, Ryoichi ; Sugimura, Yoshihisa ; Ozaki, Nobuaki ; Nagasaki, Hiroshi ; Oiso, Yutaka. / Repeated glucoprivation delayed hyperphagic responses while activating neuropeptide Y neurons in rats. In: Peptides. 2011 ; Vol. 32, No. 4. pp. 763-769.
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Ozawa, Y, Arima, H, Watanabe, M, Shimizu, H, Ito, Y, Banno, R, Sugimura, Y, Ozaki, N, Nagasaki, H & Oiso, Y 2011, 'Repeated glucoprivation delayed hyperphagic responses while activating neuropeptide Y neurons in rats', Peptides, vol. 32, no. 4, pp. 763-769. https://doi.org/10.1016/j.peptides.2010.12.009

Repeated glucoprivation delayed hyperphagic responses while activating neuropeptide Y neurons in rats. / Ozawa, Yoshiharu; Arima, Hiroshi; Watanabe, Minemori; Shimizu, Hiroshi; Ito, Yoshihiro; Banno, Ryoichi; Sugimura, Yoshihisa; Ozaki, Nobuaki; Nagasaki, Hiroshi; Oiso, Yutaka.

In: Peptides, Vol. 32, No. 4, 01.04.2011, p. 763-769.

Research output: Contribution to journalArticle

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T1 - Repeated glucoprivation delayed hyperphagic responses while activating neuropeptide Y neurons in rats

AU - Ozawa, Yoshiharu

AU - Arima, Hiroshi

AU - Watanabe, Minemori

AU - Shimizu, Hiroshi

AU - Ito, Yoshihiro

AU - Banno, Ryoichi

AU - Sugimura, Yoshihisa

AU - Ozaki, Nobuaki

AU - Nagasaki, Hiroshi

AU - Oiso, Yutaka

PY - 2011/4/1

Y1 - 2011/4/1

N2 - It is well known that glucoprivation induces the release of counterregulatory hormones such as glucagon, and that the response is attenuated when the stimuli are repeated. Glucoprivation also activates orexigenic neurons and induces hyperphagic responses, although it remains unclear whether these responses are attenuated in repeated glucoprivation. In this study, we examined time course changes in feeding as well as activities of orexigenic neuropeptide Y (NPY) neurons in repeated glucoprivation in rats. Either 2-deoxy-d-glucose (2DG), which blocks glucose utilization, or isotonic saline (control) was injected subcutaneously to rats for 14 days, and food consumption for 1 and 2 h after injection was monitored throughout the experiment. While 2DG injection induced robust feeding responses during the first 1 h after injection, the response was gradually attenuated and the food consumption was significantly less on days 12-14 compared to that on day 1. On the other hand, food consumption during 2 h after 2DG injection was not changed significantly for 14 days. The transcriptional activities of NPY neurons in the arcuate nucleus and C1/A1 region of the hindbrain, measured by intronic in situ hybridization, were significantly enhanced after repeated 2DG injection for 14 days, while the feeding responses to intracerebroventricular injection of NPY were significantly less in the 2DG-repeated group compared to the saline-repeated group. It is thus demonstrated that repeated glucoprivation delayed hyperphagic responses while activating NPY neurons in rats. Our data also suggest that decreased feeding responses to NPY might be at least partially responsible for the delayed response.

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