Reprint of

Chromogranin A: A new proposal for trafficking, processing and induction of granule biogenesis

Hisatsugu Koshimizu, Taeyoon Kim, Niamh X. Cawley, Y. Peng Loh

Research output: Contribution to journalArticle

5 Citations (Scopus)

Abstract

Chromogranin A (CgA), a member of the granin family serves several important cell biological roles in (neuro)endocrine cells which are summarized in this review. CgA is a "prohormone" that is synthesized at the rough endoplasmic reticulum and transported into the cisternae of this organelle via its signal peptide. It is then trafficked to the Golgi complex and then to the trans-Golgi network (TGN) where CgA aggregates at low pH in the presence of calcium. The CgA aggregates provide the physical driving force to induce budding of the TGN membrane resulting in dense core granule (DCG) formation. Within the granule, a small amount of the CgA is processed to bioactive peptides, including a predicted C-terminal peptide, serpinin. Upon stimulation, DCGs undergo exocytosis and CgA and its derived peptides are released. Serpinin, acting extracellularly is able to signal the increase in transcription of a serine protease inhibitor, protease nexin-1 (PN-1) that protects DCG proteins against degradation in the Golgi complex, which then enhances DCG biogenesis to replenish those that were released. Thus CgA and its derived peptide, serpinin, plays a significant role in granule formation and regulation of granule biogenesis, respectively, in (neuro) endocrine cells.

Original languageEnglish
Pages (from-to)95-101
Number of pages7
JournalRegulatory Peptides
Volume165
Issue number1
DOIs
Publication statusPublished - 30-11-2010

Fingerprint

Chromogranin A
Processing
trans-Golgi Network
Peptides
Endocrine Cells
Golgi Apparatus
Protease Nexins
Cells
Chromogranins
Serine Proteinase Inhibitors
Rough Endoplasmic Reticulum
Exocytosis
Transcription
Protein Sorting Signals
Organelles
Proteolysis
Calcium
Membranes
Degradation

All Science Journal Classification (ASJC) codes

  • Biochemistry
  • Clinical Biochemistry
  • Endocrinology
  • Physiology
  • Cellular and Molecular Neuroscience

Cite this

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Reprint of : Chromogranin A: A new proposal for trafficking, processing and induction of granule biogenesis. / Koshimizu, Hisatsugu; Kim, Taeyoon; Cawley, Niamh X.; Loh, Y. Peng.

In: Regulatory Peptides, Vol. 165, No. 1, 30.11.2010, p. 95-101.

Research output: Contribution to journalArticle

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