Reversal of streptozotocin-induced hyperglycemia by continuous supply of betacellulin in mice

Yoritsuna Yamamoto, Satoko Yamada, Tsutomu Kodera, Akemi Hara, Kazuo Motoyoshi, Yuji Tanaka, Tadahiro Nagaoka, Masaharu Seno, Itaru Kojima

Research output: Contribution to journalArticle

8 Citations (Scopus)

Abstract

Previous studies have shown the efficacy of betacellulin (BTC) to promote β-cell regeneration. Because of its short half-life, however, the effect of BTC may have been underestimated. This study was conducted to assess the effect of continuous administration of BTC on β-cell regeneration. Adenovirus vectors encoding proBTC (Ad-proBTC) and mature BTC (Ad-mBTC) were prepared, and the efficacy of secretion of BTC was compared in AML12 hepatocytes. When AML12 cells were infected with Ad-proBTC or Ad-mBTC, cells infected with Ad-mBTC secreted considerably larger amount of BTC. We then infused Ad-mBTC into the mouse tail vein. Expression of BTC was detected in the liver for at least 21 days, and serum BTC was maintained at approximately 1 ng/ml for 7 days. When Ad-mBTC was infused immediately after administration of STZ (170 mg/kg), elevation of the plasma glucose induced by STZ was markedly inhibited, and the plasma glucose concentration remained at less than 200 mg/dl for 21 days. The insulin content and the β-cell mass were significantly increased in Ad-mBTC-infused mice. These results indicate that continuous administration of BTC is quite effective in promoting regeneration of β-cells.

Original languageEnglish
Pages (from-to)173-179
Number of pages7
JournalGrowth Factors
Volume26
Issue number4
DOIs
Publication statusPublished - 01-12-2008

Fingerprint

Streptozocin
Hyperglycemia
Regeneration
Adenoviridae
Plasmas
Betacellulin
Glucose
Liver
Half-Life
Tail
Hepatocytes
Veins
Insulin
Serum

All Science Journal Classification (ASJC) codes

  • Endocrinology
  • Clinical Biochemistry
  • Cell Biology

Cite this

Yamamoto, Y., Yamada, S., Kodera, T., Hara, A., Motoyoshi, K., Tanaka, Y., ... Kojima, I. (2008). Reversal of streptozotocin-induced hyperglycemia by continuous supply of betacellulin in mice. Growth Factors, 26(4), 173-179. https://doi.org/10.1080/08977190802136854
Yamamoto, Yoritsuna ; Yamada, Satoko ; Kodera, Tsutomu ; Hara, Akemi ; Motoyoshi, Kazuo ; Tanaka, Yuji ; Nagaoka, Tadahiro ; Seno, Masaharu ; Kojima, Itaru. / Reversal of streptozotocin-induced hyperglycemia by continuous supply of betacellulin in mice. In: Growth Factors. 2008 ; Vol. 26, No. 4. pp. 173-179.
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abstract = "Previous studies have shown the efficacy of betacellulin (BTC) to promote β-cell regeneration. Because of its short half-life, however, the effect of BTC may have been underestimated. This study was conducted to assess the effect of continuous administration of BTC on β-cell regeneration. Adenovirus vectors encoding proBTC (Ad-proBTC) and mature BTC (Ad-mBTC) were prepared, and the efficacy of secretion of BTC was compared in AML12 hepatocytes. When AML12 cells were infected with Ad-proBTC or Ad-mBTC, cells infected with Ad-mBTC secreted considerably larger amount of BTC. We then infused Ad-mBTC into the mouse tail vein. Expression of BTC was detected in the liver for at least 21 days, and serum BTC was maintained at approximately 1 ng/ml for 7 days. When Ad-mBTC was infused immediately after administration of STZ (170 mg/kg), elevation of the plasma glucose induced by STZ was markedly inhibited, and the plasma glucose concentration remained at less than 200 mg/dl for 21 days. The insulin content and the β-cell mass were significantly increased in Ad-mBTC-infused mice. These results indicate that continuous administration of BTC is quite effective in promoting regeneration of β-cells.",
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Yamamoto, Y, Yamada, S, Kodera, T, Hara, A, Motoyoshi, K, Tanaka, Y, Nagaoka, T, Seno, M & Kojima, I 2008, 'Reversal of streptozotocin-induced hyperglycemia by continuous supply of betacellulin in mice', Growth Factors, vol. 26, no. 4, pp. 173-179. https://doi.org/10.1080/08977190802136854

Reversal of streptozotocin-induced hyperglycemia by continuous supply of betacellulin in mice. / Yamamoto, Yoritsuna; Yamada, Satoko; Kodera, Tsutomu; Hara, Akemi; Motoyoshi, Kazuo; Tanaka, Yuji; Nagaoka, Tadahiro; Seno, Masaharu; Kojima, Itaru.

In: Growth Factors, Vol. 26, No. 4, 01.12.2008, p. 173-179.

Research output: Contribution to journalArticle

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AU - Yamamoto, Yoritsuna

AU - Yamada, Satoko

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AU - Motoyoshi, Kazuo

AU - Tanaka, Yuji

AU - Nagaoka, Tadahiro

AU - Seno, Masaharu

AU - Kojima, Itaru

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Yamamoto Y, Yamada S, Kodera T, Hara A, Motoyoshi K, Tanaka Y et al. Reversal of streptozotocin-induced hyperglycemia by continuous supply of betacellulin in mice. Growth Factors. 2008 Dec 1;26(4):173-179. https://doi.org/10.1080/08977190802136854