Reverse genetics system of rotaviruses: development and application for analysis of VP4 spike protein

Research output: Contribution to journalArticle

Abstract

The rotavirus genome is composed of 11 gene segments of double-stranded (ds)RNA. Reverse genetics is the powerful and ideal methodology for the molecular analysis of virus biology, which enables the virus genome to be artificially manipulated. Although reverse genetics systems exist for nearly all major groups of RNA viruses, development of such a system for rotaviruses is more challenging owing in part to the technical complexity of manipulation of their multi-segmented genome. A breakthrough in the field of rotavirus reverse genetics came in 2006, when we established the first reverse genetics system for rotaviruses, which is a partially plasmid-based system that permits replacement of a viral gene segment with the aid of a helper virus. Although this helper virus-driven system is technically limited and gives low levels of recombinant viruses, it allows alteration of the rotavirus genome, thus contributing to our understanding of these medically important viruses. In this review, I describe the development and application of our rotavirus reverse genetics system, and its future perspectives.

Original languageEnglish
Pages (from-to)103-112
Number of pages10
JournalUirusu
Volume63
Issue number1
DOIs
Publication statusPublished - 01-01-2013

Fingerprint

Reverse Genetics
Rotavirus
Genome
Helper Viruses
Viruses
Proteins
Double-Stranded RNA
Viral Genes
RNA Viruses
Plasmids
Genes

All Science Journal Classification (ASJC) codes

  • Medicine(all)

Cite this

@article{c4beb4ab8c8d4b908c6407173571523b,
title = "Reverse genetics system of rotaviruses: development and application for analysis of VP4 spike protein",
abstract = "The rotavirus genome is composed of 11 gene segments of double-stranded (ds)RNA. Reverse genetics is the powerful and ideal methodology for the molecular analysis of virus biology, which enables the virus genome to be artificially manipulated. Although reverse genetics systems exist for nearly all major groups of RNA viruses, development of such a system for rotaviruses is more challenging owing in part to the technical complexity of manipulation of their multi-segmented genome. A breakthrough in the field of rotavirus reverse genetics came in 2006, when we established the first reverse genetics system for rotaviruses, which is a partially plasmid-based system that permits replacement of a viral gene segment with the aid of a helper virus. Although this helper virus-driven system is technically limited and gives low levels of recombinant viruses, it allows alteration of the rotavirus genome, thus contributing to our understanding of these medically important viruses. In this review, I describe the development and application of our rotavirus reverse genetics system, and its future perspectives.",
author = "Satoshi Komoto",
year = "2013",
month = "1",
day = "1",
doi = "10.2222/jsv.63.103",
language = "English",
volume = "63",
pages = "103--112",
journal = "Uirusu. Journal of virology",
issn = "0042-6857",
publisher = "Nihon Uirusu Gakkal/Japanese Society for Virology",
number = "1",

}

Reverse genetics system of rotaviruses : development and application for analysis of VP4 spike protein. / Komoto, Satoshi.

In: Uirusu, Vol. 63, No. 1, 01.01.2013, p. 103-112.

Research output: Contribution to journalArticle

TY - JOUR

T1 - Reverse genetics system of rotaviruses

T2 - development and application for analysis of VP4 spike protein

AU - Komoto, Satoshi

PY - 2013/1/1

Y1 - 2013/1/1

N2 - The rotavirus genome is composed of 11 gene segments of double-stranded (ds)RNA. Reverse genetics is the powerful and ideal methodology for the molecular analysis of virus biology, which enables the virus genome to be artificially manipulated. Although reverse genetics systems exist for nearly all major groups of RNA viruses, development of such a system for rotaviruses is more challenging owing in part to the technical complexity of manipulation of their multi-segmented genome. A breakthrough in the field of rotavirus reverse genetics came in 2006, when we established the first reverse genetics system for rotaviruses, which is a partially plasmid-based system that permits replacement of a viral gene segment with the aid of a helper virus. Although this helper virus-driven system is technically limited and gives low levels of recombinant viruses, it allows alteration of the rotavirus genome, thus contributing to our understanding of these medically important viruses. In this review, I describe the development and application of our rotavirus reverse genetics system, and its future perspectives.

AB - The rotavirus genome is composed of 11 gene segments of double-stranded (ds)RNA. Reverse genetics is the powerful and ideal methodology for the molecular analysis of virus biology, which enables the virus genome to be artificially manipulated. Although reverse genetics systems exist for nearly all major groups of RNA viruses, development of such a system for rotaviruses is more challenging owing in part to the technical complexity of manipulation of their multi-segmented genome. A breakthrough in the field of rotavirus reverse genetics came in 2006, when we established the first reverse genetics system for rotaviruses, which is a partially plasmid-based system that permits replacement of a viral gene segment with the aid of a helper virus. Although this helper virus-driven system is technically limited and gives low levels of recombinant viruses, it allows alteration of the rotavirus genome, thus contributing to our understanding of these medically important viruses. In this review, I describe the development and application of our rotavirus reverse genetics system, and its future perspectives.

UR - http://www.scopus.com/inward/record.url?scp=84927170719&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84927170719&partnerID=8YFLogxK

U2 - 10.2222/jsv.63.103

DO - 10.2222/jsv.63.103

M3 - Article

C2 - 24769586

AN - SCOPUS:84927170719

VL - 63

SP - 103

EP - 112

JO - Uirusu. Journal of virology

JF - Uirusu. Journal of virology

SN - 0042-6857

IS - 1

ER -