RFP is a DNA binding protein associated with the nuclear matrix

Takeshi Isomura, Keiko Tamiya-koizumi, Motoshi Suzuki, Shonen Yoshida, Masahiko Taniguchi, Mutsushi Matsuyama, Takeo Ishigaki, Sadayuki Sakuma, Masahide Takahashi

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49 Citations (Scopus)

Abstract

We reported that the RFP gene encodes a protein with putative zinc finger domains and was involved In the activation of the ret proto-oncogene. To further characterize the RFP protein, we developed a polyclonal antibody against the product synthesized from a fragment of the RFP cDNA expressed in Escherichia coll. Western blot analysis showed that RFP was identified as a 58 kDa protein In cell lysates from four human and rodent cell lines and from mouse test is. In addition, a unique 68 kDa protein was detected in the testis. Using AH7974 (rat ascites hepatoma) and Raji (human Burkttt lymphoma) cells, we demonstrated strong association of RFP with the nuclear matrix. Furthermore, RFP solubilized from the nuclear matrix had DNA-bindlng activity although It appears to bind more preferentially to double-stranded DNA than to single-stranded DNA. These results thus suggest that RFP may play a role in molecular processes which occur in the nuclear matrix.

Original languageEnglish
Pages (from-to)5305-5310
Number of pages6
JournalNucleic Acids Research
Volume20
Issue number20
DOIs
Publication statusPublished - 25-10-1992
Externally publishedYes

All Science Journal Classification (ASJC) codes

  • Genetics

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  • Cite this

    Isomura, T., Tamiya-koizumi, K., Suzuki, M., Yoshida, S., Taniguchi, M., Matsuyama, M., Ishigaki, T., Sakuma, S., & Takahashi, M. (1992). RFP is a DNA binding protein associated with the nuclear matrix. Nucleic Acids Research, 20(20), 5305-5310. https://doi.org/10.1093/nar/20.20.5305