Risperidone, an atypical antipsychotic, affects the mRNA expressions of monoamine oxidase type B and vesicular monoamine transporter-2 in rat brain

Miyuki Ota, Akira Nakashima, Keiji Mori, Toshihiko Hamanaka, Akira Ota

Research output: Contribution to journalArticle

Abstract

To determine if the antagonism of serotonin 5HT(2A) receptors and dopamine D2 receptors by risperidone alters the mRNAs encoding the transporters and enzymes that are involved in re-uptake of serotonin and dopamine and in the degradation and refilling of secretory vesicles, we employed semi-quantitative RT-PCR on rats given risperidone. The rats were given twice daily a subcutaneous injection of either 0.3% tartaric acid (vehicle) or risperidone (0.5 mg/kg) for 21 days and sacrificed one hour after the last injection. Risperidone enhanced the level of monoamine oxidase type B (MAO-B) mRNA in the brainstem (P < 0.05) and that of vesicular monoamine transporter-2 (VMAT2) mRNA in the hypothalamus (P < 0.05) when these levels were compared with the control ones, whereas there were no significant group differences in other mRNAs examined. The antagonism of 5HT(2A) and D2 receptors may thus affect the monoamine levels by regulating mRNAs encoding MAO-B and VMAT2.

Original languageEnglish
Pages (from-to)197-216
Number of pages20
JournalBiogenic Amines
Volume15
Issue number2
Publication statusPublished - 01-01-1999

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Vesicular Monoamine Transport Proteins
Risperidone
Monoamine Oxidase
Antipsychotic Agents
Messenger RNA
Brain
Receptor, Serotonin, 5-HT2A
Dopamine D2 Receptors
Secretory Vesicles
Subcutaneous Injections
Hypothalamus
Brain Stem
Dopamine
Serotonin
Polymerase Chain Reaction
Injections
Enzymes

All Science Journal Classification (ASJC) codes

  • Neuroscience(all)
  • Pharmacology

Cite this

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abstract = "To determine if the antagonism of serotonin 5HT(2A) receptors and dopamine D2 receptors by risperidone alters the mRNAs encoding the transporters and enzymes that are involved in re-uptake of serotonin and dopamine and in the degradation and refilling of secretory vesicles, we employed semi-quantitative RT-PCR on rats given risperidone. The rats were given twice daily a subcutaneous injection of either 0.3{\%} tartaric acid (vehicle) or risperidone (0.5 mg/kg) for 21 days and sacrificed one hour after the last injection. Risperidone enhanced the level of monoamine oxidase type B (MAO-B) mRNA in the brainstem (P < 0.05) and that of vesicular monoamine transporter-2 (VMAT2) mRNA in the hypothalamus (P < 0.05) when these levels were compared with the control ones, whereas there were no significant group differences in other mRNAs examined. The antagonism of 5HT(2A) and D2 receptors may thus affect the monoamine levels by regulating mRNAs encoding MAO-B and VMAT2.",
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Risperidone, an atypical antipsychotic, affects the mRNA expressions of monoamine oxidase type B and vesicular monoamine transporter-2 in rat brain. / Ota, Miyuki; Nakashima, Akira; Mori, Keiji; Hamanaka, Toshihiko; Ota, Akira.

In: Biogenic Amines, Vol. 15, No. 2, 01.01.1999, p. 197-216.

Research output: Contribution to journalArticle

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T1 - Risperidone, an atypical antipsychotic, affects the mRNA expressions of monoamine oxidase type B and vesicular monoamine transporter-2 in rat brain

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