Ritanserin, a serotonin-2 receptor antagonist, inhibits functional recovery after cerebral infarction

Research output: Contribution to journalArticle

Abstract

It has been suggested that serotonin (5-HT) may be implicated in functional recovery after stroke; however, the underlying molecular mechanisms remain unknown. Here, the role of 5-HT was verified using ritanserin, a potent 5-HT 2A receptor antagonist, and protein expression and modification were analyzed to further understand the association between paralysis recovery and molecular mechanisms in the brain. Experimental cerebral cortex infarctions were induced by photothrombosis in rats. Voluntary exercise was initiated 2 days after surgery. Motor performance was then measured using the rotarod test. Differences in protein expression and phosphorylation in the perilesional cortex were analyzed using western blot. In behavioral evaluations, performance in the rotarod test was significantly increased by exercise. However, there was a significantly lower value in time until falling after combined exercise and ritanserin administration compared with that of exercise alone. Protein expression analysis revealed that phosphorylation of protein kinase C (PKC) α, PKCϵ, and growth-associated protein 43 (GAP43) was significantly upregulated by exercise. These effects were attenuated by ritanserin administration. These data suggest that 5-HT may be related to the underlying mechanisms of exercise-dependent paralysis recovery, that is, exercise-dependent plasticity through the phosphorylation of PKC and GAP43.

Original languageEnglish
Pages (from-to)54-58
Number of pages5
JournalNeuroReport
Volume29
Issue number1
DOIs
Publication statusPublished - 03-01-2018

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Serotonin 5-HT2 Receptors
Ritanserin
Serotonin Antagonists
Cerebral Infarction
Serotonin
Rotarod Performance Test
GAP-43 Protein
Phosphorylation
Paralysis
Protein Kinase C
Receptor, Serotonin, 5-HT2A
Proteins
Ambulatory Surgical Procedures
Cerebral Cortex
Western Blotting
Stroke
Brain

All Science Journal Classification (ASJC) codes

  • Neuroscience(all)

Cite this

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Ritanserin, a serotonin-2 receptor antagonist, inhibits functional recovery after cerebral infarction. / Mizutani, Kenmei; Sonoda, Shigeru; Wakita, Hideaki.

In: NeuroReport, Vol. 29, No. 1, 03.01.2018, p. 54-58.

Research output: Contribution to journalArticle

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