TY - JOUR
T1 - RNA-sequencing based microrna expression signature of colorectal cancer
T2 - The impact of oncogenic targets regulated by miR-490-3p
AU - Hozaka, Yuto
AU - Kita, Yoshiaki
AU - Yasudome, Ryutaro
AU - Tanaka, Takako
AU - Wada, Masumi
AU - Idichi, Tetsuya
AU - Tanabe, Kan
AU - Asai, Shunichi
AU - Moriya, Shogo
AU - Toda, Hiroko
AU - Mori, Shinichiro
AU - Kurahara, Hiroshi
AU - Ohtsuka, Takao
AU - Seki, Naohiko
N1 - Publisher Copyright:
© 2021 by the authors. Licensee MDPI, Basel, Switzerland.
PY - 2021/9
Y1 - 2021/9
N2 - To elucidate novel aspects of the molecular pathogenesis of colorectal cancer (CRC), we have created a new microRNA (miRNA) expression signature based on RNA-sequencing. Analysis of the signature showed that 84 miRNAs were upregulated, and 70 were downregulated in CRC tissues. Interestingly, our signature indicated that both guide and passenger strands of some miRNAs were significantly dysregulated in CRC tissues. These findings support our earlier data demonstrating the involvement of miRNA passenger strands in cancer pathogenesis. Our study focused on downregulated miR-490-3p and investigated its tumor-suppressive function in CRC cells. We successfully identified a total of 38 putative oncogenic targets regulated by miR-490-3p in CRC cells. Among these targets, the expression of three genes (IRAK1: p = 0.0427, FUT1: p = 0.0468, and GPRIN2: p = 0.0080) significantly predicted 5-year overall survival of CRC patients. Moreover, we analyzed the direct regulation of IRAK1 by miR-490-3p, and its resultant oncogenic function in CRC cells. Thus, we have clarified a part of the molecular pathway of CRC based on the action of tumor-suppressive miR-490-3p. This new miRNA expression signature of CRC will be a useful tool for elucidating new molecular pathogenesis in this disease.
AB - To elucidate novel aspects of the molecular pathogenesis of colorectal cancer (CRC), we have created a new microRNA (miRNA) expression signature based on RNA-sequencing. Analysis of the signature showed that 84 miRNAs were upregulated, and 70 were downregulated in CRC tissues. Interestingly, our signature indicated that both guide and passenger strands of some miRNAs were significantly dysregulated in CRC tissues. These findings support our earlier data demonstrating the involvement of miRNA passenger strands in cancer pathogenesis. Our study focused on downregulated miR-490-3p and investigated its tumor-suppressive function in CRC cells. We successfully identified a total of 38 putative oncogenic targets regulated by miR-490-3p in CRC cells. Among these targets, the expression of three genes (IRAK1: p = 0.0427, FUT1: p = 0.0468, and GPRIN2: p = 0.0080) significantly predicted 5-year overall survival of CRC patients. Moreover, we analyzed the direct regulation of IRAK1 by miR-490-3p, and its resultant oncogenic function in CRC cells. Thus, we have clarified a part of the molecular pathway of CRC based on the action of tumor-suppressive miR-490-3p. This new miRNA expression signature of CRC will be a useful tool for elucidating new molecular pathogenesis in this disease.
KW - Colorectal cancer
KW - Expression signature
KW - IRAK1
KW - MiR-490-3p
KW - MicroRNA
KW - RNA-sequencing
KW - Tumor-suppressor
UR - https://www.scopus.com/pages/publications/85114742129
UR - https://www.scopus.com/pages/publications/85114742129#tab=citedBy
U2 - 10.3390/ijms22189876
DO - 10.3390/ijms22189876
M3 - Article
C2 - 34576039
AN - SCOPUS:85114742129
SN - 1661-6596
VL - 22
JO - International journal of molecular sciences
JF - International journal of molecular sciences
IS - 18
M1 - 9876
ER -
