RobA-induced multiple antibiotic resistance largely depends on the activation of the AcrAB efflux

Toshihiko Tanaka, Toshinobu Horii, Keigo Shibayama, Kumiko Sato, Shinji Ohsuka, Yoshichika Arakawa, Ken Ichi Yamaki, Kenzo Takagi, Michio Ohta

Research output: Contribution to journalArticlepeer-review

29 Citations (Scopus)

Abstract

RobA is a member of the XyIS/AraC subfamily of DNA binding proteins, and when overexpressed, it induces multiple antibiotic resistance in Escherichia coli. In this study, we introduced a multicopy robA plasmid (pMEP1) and its derivative into OmpF mutants and an AcrAB-deficient mutant. We found that a decrease in susceptibility to multiple antibiotics in these OmpF mutants when pMEP1 was introduced did not depend on OmpF porin expression. Interestingly, a ΔompF mutant (TK007) became more sensitive when pMEP1 was introduced. Moreover, no effect of RobA on the induction of multiple antibiotic resistance in an acrA1- mutant was observed. Therefore, we conclude that the multiple antibiotic resistance induced by the overexpression of RobA largely depends on the activation of the AcrAB efflux, as well as the activation of micF.

Original languageEnglish
Pages (from-to)697-702
Number of pages6
JournalMICROBIOLOGY and IMMUNOLOGY
Volume41
Issue number9
DOIs
Publication statusPublished - 1997
Externally publishedYes

All Science Journal Classification (ASJC) codes

  • Microbiology
  • Immunology
  • Virology

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