TY - JOUR
T1 - RobA-induced multiple antibiotic resistance largely depends on the activation of the AcrAB efflux
AU - Tanaka, Toshihiko
AU - Horii, Toshinobu
AU - Shibayama, Keigo
AU - Sato, Kumiko
AU - Ohsuka, Shinji
AU - Arakawa, Yoshichika
AU - Yamaki, Ken Ichi
AU - Takagi, Kenzo
AU - Ohta, Michio
PY - 1997
Y1 - 1997
N2 - RobA is a member of the XyIS/AraC subfamily of DNA binding proteins, and when overexpressed, it induces multiple antibiotic resistance in Escherichia coli. In this study, we introduced a multicopy robA plasmid (pMEP1) and its derivative into OmpF mutants and an AcrAB-deficient mutant. We found that a decrease in susceptibility to multiple antibiotics in these OmpF mutants when pMEP1 was introduced did not depend on OmpF porin expression. Interestingly, a ΔompF mutant (TK007) became more sensitive when pMEP1 was introduced. Moreover, no effect of RobA on the induction of multiple antibiotic resistance in an acrA1- mutant was observed. Therefore, we conclude that the multiple antibiotic resistance induced by the overexpression of RobA largely depends on the activation of the AcrAB efflux, as well as the activation of micF.
AB - RobA is a member of the XyIS/AraC subfamily of DNA binding proteins, and when overexpressed, it induces multiple antibiotic resistance in Escherichia coli. In this study, we introduced a multicopy robA plasmid (pMEP1) and its derivative into OmpF mutants and an AcrAB-deficient mutant. We found that a decrease in susceptibility to multiple antibiotics in these OmpF mutants when pMEP1 was introduced did not depend on OmpF porin expression. Interestingly, a ΔompF mutant (TK007) became more sensitive when pMEP1 was introduced. Moreover, no effect of RobA on the induction of multiple antibiotic resistance in an acrA1- mutant was observed. Therefore, we conclude that the multiple antibiotic resistance induced by the overexpression of RobA largely depends on the activation of the AcrAB efflux, as well as the activation of micF.
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U2 - 10.1111/j.1348-0421.1997.tb01913.x
DO - 10.1111/j.1348-0421.1997.tb01913.x
M3 - Article
C2 - 9343820
AN - SCOPUS:0030924112
SN - 0385-5600
VL - 41
SP - 697
EP - 702
JO - MICROBIOLOGY and IMMUNOLOGY
JF - MICROBIOLOGY and IMMUNOLOGY
IS - 9
ER -