TY - JOUR
T1 - Role and mechanism of U1-independent pre-mRNA splicing in the regulation of alternative splicing
AU - Fukumura, Kazuhiro
AU - Inoue, Kunio
N1 - Funding Information:
We thank Rie Kusakabe for comments on the manuscript. We are also grateful to the members of Inoue Laboratory (Kobe University), Tani Laboratory (Kumamoto University) and Ohno Laboratory (Institute for Virus Research, Kyoto University) for stimulating discussion and support. Our work was supported by Grants-in-Aid for Scientific Research from JSPS and MEXT and in part by the Mitsubishi Foundation.
PY - 2009
Y1 - 2009
N2 - In metazoan organisms, alternative splicing is a central mechanism for the regulation of gene expression. However, many questions remain about the underlying molecular mechanisms. Our recent work suggests that U1 snRNP-independent pre-mRNA splicing occurs in humans, which contributes to the regulation of alternative splicing. So far it has been reported that several pre-mRNAs were spliced efficiently in a U1 snRNP-independent manner in vitro. Although the molecular mechanism and functional significance of U1-independent pre-mRNA splicing are not well understood, a model of how the 5′ splice site is recognized U1-independently has been proposed. In this review, we first overview a model in which the 5′ splice site is recognized by SR proteins and U6 snRNA. We then discuss our novel model and the functional significance of U1-independent pre-mRNA splicing in the regulation of alternative splicing, based on our recent work.
AB - In metazoan organisms, alternative splicing is a central mechanism for the regulation of gene expression. However, many questions remain about the underlying molecular mechanisms. Our recent work suggests that U1 snRNP-independent pre-mRNA splicing occurs in humans, which contributes to the regulation of alternative splicing. So far it has been reported that several pre-mRNAs were spliced efficiently in a U1 snRNP-independent manner in vitro. Although the molecular mechanism and functional significance of U1-independent pre-mRNA splicing are not well understood, a model of how the 5′ splice site is recognized U1-independently has been proposed. In this review, we first overview a model in which the 5′ splice site is recognized by SR proteins and U6 snRNA. We then discuss our novel model and the functional significance of U1-independent pre-mRNA splicing in the regulation of alternative splicing, based on our recent work.
KW - 5′ splice site
KW - Alternative splicing
KW - Fox-1
KW - U1 snRNP
KW - U1-independent pre-mRNA splicing
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U2 - 10.4161/rna.6.4.9318
DO - 10.4161/rna.6.4.9318
M3 - Review article
C2 - 19667760
AN - SCOPUS:70349609214
SN - 1547-6286
VL - 6
JO - RNA Biology
JF - RNA Biology
IS - 4
ER -