Role of a sialyl lewisx-like epitope selectively expressed on vascular endothelial cells in local skin inflammation of the rat

Toshiyuki Akahori, Yukio Yuzawa, Kazuhiro Nishikawa, Takuya Tamatani, Reiji Kannagi, Masayuki Miyasaka, Hidechika Okada, Nigishi Hotta, Seiichi Matsuo

Research output: Contribution to journalArticle

19 Citations (Scopus)

Abstract

The role of the inducible L-selectin ligand was studied in complement-dependent acute dermatitis in rats. Although mAbs against typical sialyl Lewisx (CSLEX-1 and SNH-3) did not react with skin venules, a sialyl Lewisx-like epitope defined by mAb 2H5 (2H5-Ag) was de novo expressed on the endothelial cells of skin venules in the area of inflammation. Expression of 2H5-Ag increased concomitantly with the progression of inflammation. 2H5-Ag was identified at the 75-, 150-, and 180-kDa bands when inflammatory skin tissue was analyzed by Western blotting. In contrast, P- and E-selectins were not detectable. The role of 2H5-Ag in this model was studied in in vitro and in vivo methods. First, 2H5 was i.v. injected 15 min before induction of dermatitis. 2H5 bound to skin venules and significantly reduced the neutrophil infiltration and plasma protein leakage. In contrast, CSLEX-1, mAb ARP2-4 (P-selectin blocker), or mAb ARE-5 (E-selectin blocker) had no effects. Second, adhesion of isolated rat neutrophils to the inflammatory skin section was inhibited significantly when the sections, but not neutrophils, were preincubated with 2H5. Third, fluorescein-labeled normal rat neutrophils were injected into a rat 10 h after induction of dermatitis. The number of labeled neutrophils infiltrated into the inflammatory site was reduced significantly when they were preincubated with HRL-3 (blocking mAb against rat L-selectin), but not with 2H5 or HRL-4 (nonblocking mAb against rat L-selectin). These data show that de novo expressed 2H5-Ag/L-selectin adhesion pathway contributes to the development of acute complement-dependent inflammation in the skin.

Original languageEnglish
Pages (from-to)5384-5392
Number of pages9
JournalJournal of Immunology
Volume158
Issue number11
Publication statusPublished - 01-12-1997
Externally publishedYes

Fingerprint

L-Selectin
Epitopes
Endothelial Cells
Inflammation
Skin
Venules
Neutrophils
Dermatitis
P-Selectin
E-Selectin
Neutrophil Infiltration
Fluorescein
Blood Proteins
Western Blotting
Ligands

All Science Journal Classification (ASJC) codes

  • Immunology

Cite this

Akahori, T., Yuzawa, Y., Nishikawa, K., Tamatani, T., Kannagi, R., Miyasaka, M., ... Matsuo, S. (1997). Role of a sialyl lewisx-like epitope selectively expressed on vascular endothelial cells in local skin inflammation of the rat. Journal of Immunology, 158(11), 5384-5392.
Akahori, Toshiyuki ; Yuzawa, Yukio ; Nishikawa, Kazuhiro ; Tamatani, Takuya ; Kannagi, Reiji ; Miyasaka, Masayuki ; Okada, Hidechika ; Hotta, Nigishi ; Matsuo, Seiichi. / Role of a sialyl lewisx-like epitope selectively expressed on vascular endothelial cells in local skin inflammation of the rat. In: Journal of Immunology. 1997 ; Vol. 158, No. 11. pp. 5384-5392.
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abstract = "The role of the inducible L-selectin ligand was studied in complement-dependent acute dermatitis in rats. Although mAbs against typical sialyl Lewisx (CSLEX-1 and SNH-3) did not react with skin venules, a sialyl Lewisx-like epitope defined by mAb 2H5 (2H5-Ag) was de novo expressed on the endothelial cells of skin venules in the area of inflammation. Expression of 2H5-Ag increased concomitantly with the progression of inflammation. 2H5-Ag was identified at the 75-, 150-, and 180-kDa bands when inflammatory skin tissue was analyzed by Western blotting. In contrast, P- and E-selectins were not detectable. The role of 2H5-Ag in this model was studied in in vitro and in vivo methods. First, 2H5 was i.v. injected 15 min before induction of dermatitis. 2H5 bound to skin venules and significantly reduced the neutrophil infiltration and plasma protein leakage. In contrast, CSLEX-1, mAb ARP2-4 (P-selectin blocker), or mAb ARE-5 (E-selectin blocker) had no effects. Second, adhesion of isolated rat neutrophils to the inflammatory skin section was inhibited significantly when the sections, but not neutrophils, were preincubated with 2H5. Third, fluorescein-labeled normal rat neutrophils were injected into a rat 10 h after induction of dermatitis. The number of labeled neutrophils infiltrated into the inflammatory site was reduced significantly when they were preincubated with HRL-3 (blocking mAb against rat L-selectin), but not with 2H5 or HRL-4 (nonblocking mAb against rat L-selectin). These data show that de novo expressed 2H5-Ag/L-selectin adhesion pathway contributes to the development of acute complement-dependent inflammation in the skin.",
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Akahori, T, Yuzawa, Y, Nishikawa, K, Tamatani, T, Kannagi, R, Miyasaka, M, Okada, H, Hotta, N & Matsuo, S 1997, 'Role of a sialyl lewisx-like epitope selectively expressed on vascular endothelial cells in local skin inflammation of the rat', Journal of Immunology, vol. 158, no. 11, pp. 5384-5392.

Role of a sialyl lewisx-like epitope selectively expressed on vascular endothelial cells in local skin inflammation of the rat. / Akahori, Toshiyuki; Yuzawa, Yukio; Nishikawa, Kazuhiro; Tamatani, Takuya; Kannagi, Reiji; Miyasaka, Masayuki; Okada, Hidechika; Hotta, Nigishi; Matsuo, Seiichi.

In: Journal of Immunology, Vol. 158, No. 11, 01.12.1997, p. 5384-5392.

Research output: Contribution to journalArticle

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T1 - Role of a sialyl lewisx-like epitope selectively expressed on vascular endothelial cells in local skin inflammation of the rat

AU - Akahori, Toshiyuki

AU - Yuzawa, Yukio

AU - Nishikawa, Kazuhiro

AU - Tamatani, Takuya

AU - Kannagi, Reiji

AU - Miyasaka, Masayuki

AU - Okada, Hidechika

AU - Hotta, Nigishi

AU - Matsuo, Seiichi

PY - 1997/12/1

Y1 - 1997/12/1

N2 - The role of the inducible L-selectin ligand was studied in complement-dependent acute dermatitis in rats. Although mAbs against typical sialyl Lewisx (CSLEX-1 and SNH-3) did not react with skin venules, a sialyl Lewisx-like epitope defined by mAb 2H5 (2H5-Ag) was de novo expressed on the endothelial cells of skin venules in the area of inflammation. Expression of 2H5-Ag increased concomitantly with the progression of inflammation. 2H5-Ag was identified at the 75-, 150-, and 180-kDa bands when inflammatory skin tissue was analyzed by Western blotting. In contrast, P- and E-selectins were not detectable. The role of 2H5-Ag in this model was studied in in vitro and in vivo methods. First, 2H5 was i.v. injected 15 min before induction of dermatitis. 2H5 bound to skin venules and significantly reduced the neutrophil infiltration and plasma protein leakage. In contrast, CSLEX-1, mAb ARP2-4 (P-selectin blocker), or mAb ARE-5 (E-selectin blocker) had no effects. Second, adhesion of isolated rat neutrophils to the inflammatory skin section was inhibited significantly when the sections, but not neutrophils, were preincubated with 2H5. Third, fluorescein-labeled normal rat neutrophils were injected into a rat 10 h after induction of dermatitis. The number of labeled neutrophils infiltrated into the inflammatory site was reduced significantly when they were preincubated with HRL-3 (blocking mAb against rat L-selectin), but not with 2H5 or HRL-4 (nonblocking mAb against rat L-selectin). These data show that de novo expressed 2H5-Ag/L-selectin adhesion pathway contributes to the development of acute complement-dependent inflammation in the skin.

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