Role of CD1 antigen and NKT cells in Concanavalin A-induced hepatitis

Tadakazu Shimizu, Akihiro Matsuura

Research output: Contribution to journalArticle

Abstract

The MHC class Ib molecule, CD1, has been conserved throughout mammalian evolution. To assess the function of CD1, we investigated the Concanavalin A induced hepatitis model that shows increased CD1 expression in hepatocytes. In this hepatitis model, NKT cells were found to be numerically expanded. Cytotoxicity of hepatic mononuclear cells (MNC) was activated by CD1 expression in the target cells and was strongly inhibited by CD1 transfectant used as cold targets. Furthermore, the hepatic MNC depleted of NKT cells demonstrated lower cytotoxic activity against GD1-expressing cells. These observations suggest the possibility that NKT cells recognize CD1 and have cytotoxic activity against CD1 expressing cells. In previous reports about the mouse hepatitis model, chemical factors such as IL 4 and TNF-α production from NKT cells were thought to be very important. Our study provides new insights into the mechanism through which direct interaction of CD1 and NKT cells takes place.

Original languageEnglish
Pages (from-to)263-269
Number of pages7
JournalSapporo Medical Journal
Volume66
Issue number6
Publication statusPublished - 01-12-1997
Externally publishedYes

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CD1 Antigens
Natural Killer T-Cells
Concanavalin A
Hepatitis
Hepatocytes
Chemical Models
Interleukin-4

All Science Journal Classification (ASJC) codes

  • Medicine(all)

Cite this

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abstract = "The MHC class Ib molecule, CD1, has been conserved throughout mammalian evolution. To assess the function of CD1, we investigated the Concanavalin A induced hepatitis model that shows increased CD1 expression in hepatocytes. In this hepatitis model, NKT cells were found to be numerically expanded. Cytotoxicity of hepatic mononuclear cells (MNC) was activated by CD1 expression in the target cells and was strongly inhibited by CD1 transfectant used as cold targets. Furthermore, the hepatic MNC depleted of NKT cells demonstrated lower cytotoxic activity against GD1-expressing cells. These observations suggest the possibility that NKT cells recognize CD1 and have cytotoxic activity against CD1 expressing cells. In previous reports about the mouse hepatitis model, chemical factors such as IL 4 and TNF-α production from NKT cells were thought to be very important. Our study provides new insights into the mechanism through which direct interaction of CD1 and NKT cells takes place.",
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Role of CD1 antigen and NKT cells in Concanavalin A-induced hepatitis. / Shimizu, Tadakazu; Matsuura, Akihiro.

In: Sapporo Medical Journal, Vol. 66, No. 6, 01.12.1997, p. 263-269.

Research output: Contribution to journalArticle

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