Role of cholinergic and GABAergic neuronal systems in cycloheximide-induced amnesia in mice

Toshitaka Nabeshima, Yukihiro Noda, Kaname Itoh, Tsutomu Kameyama

Research output: Contribution to journalArticle

23 Citations (Scopus)

Abstract

The role of cholinergic and GABAergic neuronal systems on the cycloheximide (CXM)-induced amnesia was investigated using the step-down-type passive avoidance task in mice. CXM (7.5-120 mg/kg, SC) given just after the training caused amnesia (indicated by short latency to step down from the platform on the grid floor) in the retention test conducted 24 hr later in a dose-dependent fashion. In the CXM (60 mg/kg)-treated mice, a choline esterase inhibitor, physostigmine (PHY; 0.125 and 0.25 mg/kg, IP), or GABA agonists, muscimol (1 and 2 mg/kg, IP) and baclofen (6 and 12 mg/kg, IP), given just after training markedly prolonged step down latency (SDL), indicating reversal of amnesia. The antiamnesic action of PHY (0.125 mg/kg) was almost completely antagonized by a central acetylcholine antagonist, scopolamine (3 mg/kg, SC), but not by a peripheral acetylcholine antagonist, butylscopolamine (3 mg/kg, SC). Furthermore, the antiamnesic action of muscimol (2 mg/kg) was reversed by GABA antagonists, picrotoxin (0.5 mg/kg, SC) and bicuculline (0.5 mg/kg, SC), while the effect of baclofen (12 mg/kg) was reversed by picrotoxin (0.5 mg/kg), but not by bicuculline (0.5 mg/kg). These results suggest that the dysfunction of cholinergic and GABAergic neuronal systems play an important role in the CXM-induced memory impairment on the passive avoidance task.

Original languageEnglish
Pages (from-to)405-409
Number of pages5
JournalPharmacology, Biochemistry and Behavior
Volume31
Issue number2
DOIs
Publication statusPublished - 01-01-1988
Externally publishedYes

Fingerprint

Amnesia
Cycloheximide
Cholinergic Agents
Picrotoxin
Muscimol
Baclofen
Bicuculline
Cholinergic Antagonists
Butylscopolammonium Bromide
GABA Antagonists
GABA Agonists
Physostigmine
Scopolamine Hydrobromide
Esterases
Choline
Data storage equipment

All Science Journal Classification (ASJC) codes

  • Biochemistry
  • Toxicology
  • Pharmacology
  • Clinical Biochemistry
  • Biological Psychiatry
  • Behavioral Neuroscience

Cite this

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title = "Role of cholinergic and GABAergic neuronal systems in cycloheximide-induced amnesia in mice",
abstract = "The role of cholinergic and GABAergic neuronal systems on the cycloheximide (CXM)-induced amnesia was investigated using the step-down-type passive avoidance task in mice. CXM (7.5-120 mg/kg, SC) given just after the training caused amnesia (indicated by short latency to step down from the platform on the grid floor) in the retention test conducted 24 hr later in a dose-dependent fashion. In the CXM (60 mg/kg)-treated mice, a choline esterase inhibitor, physostigmine (PHY; 0.125 and 0.25 mg/kg, IP), or GABA agonists, muscimol (1 and 2 mg/kg, IP) and baclofen (6 and 12 mg/kg, IP), given just after training markedly prolonged step down latency (SDL), indicating reversal of amnesia. The antiamnesic action of PHY (0.125 mg/kg) was almost completely antagonized by a central acetylcholine antagonist, scopolamine (3 mg/kg, SC), but not by a peripheral acetylcholine antagonist, butylscopolamine (3 mg/kg, SC). Furthermore, the antiamnesic action of muscimol (2 mg/kg) was reversed by GABA antagonists, picrotoxin (0.5 mg/kg, SC) and bicuculline (0.5 mg/kg, SC), while the effect of baclofen (12 mg/kg) was reversed by picrotoxin (0.5 mg/kg), but not by bicuculline (0.5 mg/kg). These results suggest that the dysfunction of cholinergic and GABAergic neuronal systems play an important role in the CXM-induced memory impairment on the passive avoidance task.",
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Role of cholinergic and GABAergic neuronal systems in cycloheximide-induced amnesia in mice. / Nabeshima, Toshitaka; Noda, Yukihiro; Itoh, Kaname; Kameyama, Tsutomu.

In: Pharmacology, Biochemistry and Behavior, Vol. 31, No. 2, 01.01.1988, p. 405-409.

Research output: Contribution to journalArticle

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