Role of En2 in the tectal laminar formation of chick embryos

Minoru Omi, Hidekiyo Harada, Yuji Watanabe, Jun Ichi Funahashi, Harukazu Nakamura

Research output: Contribution to journalArticle

9 Citations (Scopus)

Abstract

The chick optic tectum consists of 16 laminae. Here, we report contribution of En2 to laminar formation in chick optic tecta. En2 is specifically expressed in laminae g-j of stratum griseum et fibrosum superficiale (SGFS). Misexpression of En2 resulted in disappearance of En2-expressing cells from the superficial layers (laminae a-f of SGFS), where endogenous En2 is not expressed. Misexpression of En2 before postmitotic cells had left the ventricular layer indicated that En2-misexpressing cells stopped at the laminae of endogenous En2 expression and that they did not migrate into the superficial layers. Induction of En2 misexpression using a tetracycline-inducible system after the postmitotic cells had reached superficial layers also resulted in disappearance of En2-expressing cells from the superficial layers. Time-lapse analysis showed that En2-misexpressing cells migrated back from the superficial layers towards the middle layers, where En2 is strongly expressed endogenously. Our results suggest a potential role of En2 in regulating cell migration and positioning in the tectal laminar formation.

Original languageEnglish
Pages (from-to)2131-2138
Number of pages8
JournalDevelopment (Cambridge)
Volume141
Issue number10
DOIs
Publication statusPublished - 2014

All Science Journal Classification (ASJC) codes

  • Molecular Biology
  • Developmental Biology

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    Omi, M., Harada, H., Watanabe, Y., Funahashi, J. I., & Nakamura, H. (2014). Role of En2 in the tectal laminar formation of chick embryos. Development (Cambridge), 141(10), 2131-2138. https://doi.org/10.1242/dev.102905