Role of IQGAP1, a target of the small GTPases Cdc42 and Rac1, in regulation of E-Cadherin-mediated cell-cell adhesion

Shinya Kuroda, Masaki Fukata, Masato Nakagawa, Katsuhiko Fujii, Tomoko Nakamura, Tadashi Ookubo, Ichiro Izawa, Takahiro Nagase, Nobuo Nomura, Hideki Tani, Ikuo Shoji, Yoshiharu Matsuura, Shin Yonehara, Kozo Kaibuchi

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Abstract

The small guanosine triphosphatases (GTPases) Cdc42 and Rac1 regulate E- cadherin-mediated cell-cell adhesion. IQGAP1, a target of Cdc42 and Rac1, was localized with E-cadherin and β-catenin at sites of cell-cell contact in mouse L fibroblasts expressing E-cadherin (EL cells), and interacted with E- cadherin and β-catenin both in vivo and in vitro. IQGAP1 induced the dissociation of α-catenin from a cadherin-catenin complex in vitro and in vivo. Overexpression of IQGAP1 in EL cells, but not in L cells expressing an E-cadherin-α-catenin chimeric protein, resulted in a decrease in E-cadherin- mediated cell-cell, adhesive activity. Thus, IQGAP1, acting downstream of Cdc42 and Rac1, appears to regulate cell-cell adhesion through the cadherin- catenin pathway.

Original languageEnglish
Pages (from-to)832-835
Number of pages4
JournalScience
Volume281
Issue number5378
DOIs
Publication statusPublished - 07-08-1998

All Science Journal Classification (ASJC) codes

  • General

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    Kuroda, S., Fukata, M., Nakagawa, M., Fujii, K., Nakamura, T., Ookubo, T., Izawa, I., Nagase, T., Nomura, N., Tani, H., Shoji, I., Matsuura, Y., Yonehara, S., & Kaibuchi, K. (1998). Role of IQGAP1, a target of the small GTPases Cdc42 and Rac1, in regulation of E-Cadherin-mediated cell-cell adhesion. Science, 281(5378), 832-835. https://doi.org/10.1126/science.281.5378.832