Role of latent membrane protein 1 in chronic active Epstein-Barr virus infection-derived T/NK-cell proliferation

Takuto Ito, Hidetaka Kawazu, Takayuki Murata, Seiko Iwata, Saki Arakawa, Yoshitaka Sato, Kiyotaka Kuzushima, Fumi Goshima, Hiroshi Kimura

Research output: Contribution to journalArticle

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Abstract

Epstein-Barr virus (EBV) predominantly infects B cells and causes B-cell lymphomas, such as Burkitt lymphoma and Hodgkin lymphoma. However, it also infects other types of cells, including T and natural killer (NK) cells, and causes disorders, such as chronic active EBV infection (CAEBV) and T/NK-cell lymphoma. The CAEBV is a lymphoproliferative disease with poor prognosis, where EBV-positive T or NK cells grow rapidly, although the molecular mechanisms that cause the cell expansion still remain to be elucidated. EBV-encoded latent membrane protein 1 (LMP1) is an oncogene that can transform some cell types, such as B cells and mouse fibroblasts, and thus may stimulate cell proliferation in CAEBV. Here, we examined the effect of LMP1 on EBV-negative cells using the cells conditionally expressing LMP1, and on CAEBV-derived EBV-positive cells by inhibiting the function of LMP1 using a dominant negative form of LMP1. We demonstrated that LMP1 was responsible for the increased cell proliferation in the cell lines derived from CAEBV, while LMP1 did not give any proliferative advantage to the EBV-negative cell line.

Original languageEnglish
Pages (from-to)787-795
Number of pages9
JournalCancer Medicine
Volume3
Issue number4
DOIs
Publication statusPublished - 01-01-2014

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Epstein-Barr Virus Infections
Membrane Proteins
Cell Proliferation
Human Herpesvirus 4
Natural Killer T-Cells
B-Lymphocytes
Cell Line
Burkitt Lymphoma
B-Cell Lymphoma
Hodgkin Disease
Oncogenes
Lymphoma
Fibroblasts

All Science Journal Classification (ASJC) codes

  • Oncology
  • Radiology Nuclear Medicine and imaging
  • Cancer Research

Cite this

Ito, Takuto ; Kawazu, Hidetaka ; Murata, Takayuki ; Iwata, Seiko ; Arakawa, Saki ; Sato, Yoshitaka ; Kuzushima, Kiyotaka ; Goshima, Fumi ; Kimura, Hiroshi. / Role of latent membrane protein 1 in chronic active Epstein-Barr virus infection-derived T/NK-cell proliferation. In: Cancer Medicine. 2014 ; Vol. 3, No. 4. pp. 787-795.
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Ito, T, Kawazu, H, Murata, T, Iwata, S, Arakawa, S, Sato, Y, Kuzushima, K, Goshima, F & Kimura, H 2014, 'Role of latent membrane protein 1 in chronic active Epstein-Barr virus infection-derived T/NK-cell proliferation', Cancer Medicine, vol. 3, no. 4, pp. 787-795. https://doi.org/10.1002/cam4.256

Role of latent membrane protein 1 in chronic active Epstein-Barr virus infection-derived T/NK-cell proliferation. / Ito, Takuto; Kawazu, Hidetaka; Murata, Takayuki; Iwata, Seiko; Arakawa, Saki; Sato, Yoshitaka; Kuzushima, Kiyotaka; Goshima, Fumi; Kimura, Hiroshi.

In: Cancer Medicine, Vol. 3, No. 4, 01.01.2014, p. 787-795.

Research output: Contribution to journalArticle

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AU - Kimura, Hiroshi

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