Role of lysophosphatidylcholine in eosinophil infiltration and resistance in airways

Osamu Nishiyama, Hiroaki Kume, Masashi Kondo, Yasushi Ito, Masafumi Ito, Kenichi Yamaki

Research output: Contribution to journalArticle

15 Citations (Scopus)

Abstract

1. Lysophosphatidylcholine (Lyso-PC), which is synthesized by phospholipase A2, is generally considered to induce adhesion molecules. However, little is known about the involvement of Lyso-PC in the pathogenesis of bronchial asthma. The present study was designed to examine whether pre-exposure to Lyso-PC causes eosinophil recruitment and an increase in resistance in airways. 2. Eosinophils in bronchoalveolar lavage fluid (BALF) and the airway walls were enumerated after inhalation of 0.5 mg/mL Lyso-PC to guinea-pigs for 10 min. Respiratory resistance (Rrs) was recorded continuously over 6 h after inhalation of an equi-dose of Lyso-PC for an equivalent period. 3. The proportion of eosinophils was increased from 10.7 ± 3.3 to 27.5 ± 3.1% (P < 0.0001) in BALF 6 h after inhalation of Lyso-PC, whereas the proportion of neutrophils and lymphocytes was not increased. Histological examination also showed uniform distribution of eosinophils in the airway wall of bronchi and bronchioles 6 h after inhalation of Lyso-PC. The number of eosinophils (/10 h.p.f.) in the bronchi and bronchioles was increased from 43.5 ± 16.8 to 154.8 ± 21.7 (P < 0.0001) and from 34.8 ± 0.7 to 106.0 ± 26.6 (P < 0.01), respectively. This eosinophil infiltration was similarly observed 24 h later. 4. Next, we examined the effects of eosinophil infiltration induced by Lyso-PC on Rrs. Inhalation of Lyso-PC caused a slow increase in Rrs and the percentage increase in Rrs, was 19.8 ± 1.9% (P < 0.0001) 6 h later. Eosinophil infiltration and an increase in Rrs, did not occur after inhalation of physiological saline. These phenomena induced by Lyso-PC were diminished by pretreatment with dexamethasone (6 μg/kg per day for 3 days). 5. Lysophosphatidylcholine causes eosinophil infiltration and a subsequent increase in resistance in airways. Our results indicate that Lyso-PC may be involved in the pathophysiology of bronchial asthma.

Original languageEnglish
Pages (from-to)179-184
Number of pages6
JournalClinical and Experimental Pharmacology and Physiology
Volume31
Issue number3
DOIs
Publication statusPublished - 01-03-2004

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Lysophosphatidylcholines
Airway Resistance
Eosinophils
Inhalation
Bronchioles
Bronchoalveolar Lavage Fluid
Bronchi
Asthma
Phospholipases A2
Dexamethasone
Guinea Pigs
Neutrophils
Lymphocytes

All Science Journal Classification (ASJC) codes

  • Physiology
  • Pharmacology
  • Physiology (medical)

Cite this

Nishiyama, Osamu ; Kume, Hiroaki ; Kondo, Masashi ; Ito, Yasushi ; Ito, Masafumi ; Yamaki, Kenichi. / Role of lysophosphatidylcholine in eosinophil infiltration and resistance in airways. In: Clinical and Experimental Pharmacology and Physiology. 2004 ; Vol. 31, No. 3. pp. 179-184.
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Role of lysophosphatidylcholine in eosinophil infiltration and resistance in airways. / Nishiyama, Osamu; Kume, Hiroaki; Kondo, Masashi; Ito, Yasushi; Ito, Masafumi; Yamaki, Kenichi.

In: Clinical and Experimental Pharmacology and Physiology, Vol. 31, No. 3, 01.03.2004, p. 179-184.

Research output: Contribution to journalArticle

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AU - Nishiyama, Osamu

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AU - Yamaki, Kenichi

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N2 - 1. Lysophosphatidylcholine (Lyso-PC), which is synthesized by phospholipase A2, is generally considered to induce adhesion molecules. However, little is known about the involvement of Lyso-PC in the pathogenesis of bronchial asthma. The present study was designed to examine whether pre-exposure to Lyso-PC causes eosinophil recruitment and an increase in resistance in airways. 2. Eosinophils in bronchoalveolar lavage fluid (BALF) and the airway walls were enumerated after inhalation of 0.5 mg/mL Lyso-PC to guinea-pigs for 10 min. Respiratory resistance (Rrs) was recorded continuously over 6 h after inhalation of an equi-dose of Lyso-PC for an equivalent period. 3. The proportion of eosinophils was increased from 10.7 ± 3.3 to 27.5 ± 3.1% (P < 0.0001) in BALF 6 h after inhalation of Lyso-PC, whereas the proportion of neutrophils and lymphocytes was not increased. Histological examination also showed uniform distribution of eosinophils in the airway wall of bronchi and bronchioles 6 h after inhalation of Lyso-PC. The number of eosinophils (/10 h.p.f.) in the bronchi and bronchioles was increased from 43.5 ± 16.8 to 154.8 ± 21.7 (P < 0.0001) and from 34.8 ± 0.7 to 106.0 ± 26.6 (P < 0.01), respectively. This eosinophil infiltration was similarly observed 24 h later. 4. Next, we examined the effects of eosinophil infiltration induced by Lyso-PC on Rrs. Inhalation of Lyso-PC caused a slow increase in Rrs and the percentage increase in Rrs, was 19.8 ± 1.9% (P < 0.0001) 6 h later. Eosinophil infiltration and an increase in Rrs, did not occur after inhalation of physiological saline. These phenomena induced by Lyso-PC were diminished by pretreatment with dexamethasone (6 μg/kg per day for 3 days). 5. Lysophosphatidylcholine causes eosinophil infiltration and a subsequent increase in resistance in airways. Our results indicate that Lyso-PC may be involved in the pathophysiology of bronchial asthma.

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