TY - JOUR
T1 - Role of nitric oxide in lipopolysaccharide-induced hepatic injury in D- galactosamine-sensitized mice as an experimental endotoxic shock model
AU - Morikawa, Akiko
AU - Kato, Yutaka
AU - Sugiyama, Tuyoshi
AU - Koide, Naoki
AU - Chakravortty, Dipshika
AU - Yoshida, Tomoaki
AU - Yokochi, Takashi
PY - 1999/3
Y1 - 1999/3
N2 - The role of nitric oxide (NO) in lipopolysaccharide (LPS)-induced hepatic injury was studied in D-galactosamine (D-GalN)-sensitized mice. The inducible isoform of NO synthase (iNOS) was immunohistochemically detected on hepatocytes around blood vessels in livers of mice injected with D-GalN and LPS not on hepatocytes in mice injected with D-GalN or LPS alone, although mRNA for iNOS was found in those mice. Nitrotyrosine (NT) was also found in livers of mice injected with D-GalN and LPS. The localization of NT was consistent with that of iNOS, and the time courses of NT and iNOS expression were almost the same. Expression of iNOS and NT was detected exclusively in the hepatic lesions of mice injected with D-GalN and LPS. Anti-tumor necrosis factor alpha neutralizing antibody inhibited iNOS and NT expression and hepatic injury. The results suggested that NO from iNOS may play a role in LPS-induced hepatic injury on D-GalN-sensitized mice as an experimental endotoxic shock model.
AB - The role of nitric oxide (NO) in lipopolysaccharide (LPS)-induced hepatic injury was studied in D-galactosamine (D-GalN)-sensitized mice. The inducible isoform of NO synthase (iNOS) was immunohistochemically detected on hepatocytes around blood vessels in livers of mice injected with D-GalN and LPS not on hepatocytes in mice injected with D-GalN or LPS alone, although mRNA for iNOS was found in those mice. Nitrotyrosine (NT) was also found in livers of mice injected with D-GalN and LPS. The localization of NT was consistent with that of iNOS, and the time courses of NT and iNOS expression were almost the same. Expression of iNOS and NT was detected exclusively in the hepatic lesions of mice injected with D-GalN and LPS. Anti-tumor necrosis factor alpha neutralizing antibody inhibited iNOS and NT expression and hepatic injury. The results suggested that NO from iNOS may play a role in LPS-induced hepatic injury on D-GalN-sensitized mice as an experimental endotoxic shock model.
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U2 - 10.1128/iai.67.3.1018-1024.1999
DO - 10.1128/iai.67.3.1018-1024.1999
M3 - Article
C2 - 10024538
AN - SCOPUS:0033050296
SN - 0019-9567
VL - 67
SP - 1018
EP - 1024
JO - Infection and Immunity
JF - Infection and Immunity
IS - 3
ER -
