Roles of glutamate receptor δ2 subunit (GluRδ2) and metabotropic glutamate receptor subtype 1 (mGluR1) in climbing fiber synapse elimination during postnatal cerebellar development

Kouichi Hashimoto, Ryoichi Ichikawa, Hajime Takechi, Yoshiro Inoue, Atsu Aiba, Kenji Sakimura, Masayoshi Mishina, Tsutomu Hashikawa, Arthur Konnerth, Masahiko Watanabe, Masanobu Kano

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Abstract

Climbing fiber (CF) synapse formation onto cerebellar Purkinje cells (PCs) is critically dependent on the synaptogenesis from parallel fibers (PFs), the other input to PCs. Previous studies revealed that deletion of the glutamate receptor δ2 subunit (GluRδ2) gene results in persistent multiple CF innervation of PCs with impaired PF synaptogenesis, whereas mutation of the metabotropic glutamate receptor subtype 1 (mGluR1) gene causes multiple CF innervation with normal PF synaptogenesis. We demonstrate that atypical CF-mediated EPSCs (CF-EPSCs) with slow rise times and small amplitudes coexisted with typical CF-EPSCs with fast rise times and large amplitudes in PCs from GluRδ2 mutant cerebellar slices. CF-EPSCs in mGluR1 mutant and wild-type PCs had fast rise times. Atypical slow CF responses of GluRδ2 mutant PCs were associated with voltage-dependent Ca2+ signals that were confined to PC distal dendrites. In the wild-type and mGluR1 mutant PCs, CF-induced Ca2+ signals involved both proximal and distal dendrites. Morphologically, CFs of GluRδ2 mutant mice extended to the superficial regions of the molecular layer, whereas those of wild-type and mGluR1 mutant mice did not innervate the superficial one-fifth of the molecular layer. It is therefore likely that surplus CFs of GluRδ2 mutant mice form ectopic synapses onto distal dendrites, whereas those of wild-type and mGluR1 mutant mice innervate proximal dendrites. These findings suggest that GluRδ2 is required for consolidating PF synapses and restricting CF synapses to the proximal dendrites, whereas the mGluR1-signaling pathway does not affect PF synaptogenesis but is involved in eliminating surplus CF synapses at the proximal dendrites.

Original languageEnglish
Pages (from-to)9701-9712
Number of pages12
JournalJournal of Neuroscience
Volume21
Issue number24
Publication statusPublished - 15-12-2001

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Purkinje Cells
Glutamate Receptors
Synapses
Dendrites
metabotropic glutamate receptor type 1
Genes
Mutation

All Science Journal Classification (ASJC) codes

  • Neuroscience(all)

Cite this

Hashimoto, Kouichi ; Ichikawa, Ryoichi ; Takechi, Hajime ; Inoue, Yoshiro ; Aiba, Atsu ; Sakimura, Kenji ; Mishina, Masayoshi ; Hashikawa, Tsutomu ; Konnerth, Arthur ; Watanabe, Masahiko ; Kano, Masanobu. / Roles of glutamate receptor δ2 subunit (GluRδ2) and metabotropic glutamate receptor subtype 1 (mGluR1) in climbing fiber synapse elimination during postnatal cerebellar development. In: Journal of Neuroscience. 2001 ; Vol. 21, No. 24. pp. 9701-9712.
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title = "Roles of glutamate receptor δ2 subunit (GluRδ2) and metabotropic glutamate receptor subtype 1 (mGluR1) in climbing fiber synapse elimination during postnatal cerebellar development",
abstract = "Climbing fiber (CF) synapse formation onto cerebellar Purkinje cells (PCs) is critically dependent on the synaptogenesis from parallel fibers (PFs), the other input to PCs. Previous studies revealed that deletion of the glutamate receptor δ2 subunit (GluRδ2) gene results in persistent multiple CF innervation of PCs with impaired PF synaptogenesis, whereas mutation of the metabotropic glutamate receptor subtype 1 (mGluR1) gene causes multiple CF innervation with normal PF synaptogenesis. We demonstrate that atypical CF-mediated EPSCs (CF-EPSCs) with slow rise times and small amplitudes coexisted with typical CF-EPSCs with fast rise times and large amplitudes in PCs from GluRδ2 mutant cerebellar slices. CF-EPSCs in mGluR1 mutant and wild-type PCs had fast rise times. Atypical slow CF responses of GluRδ2 mutant PCs were associated with voltage-dependent Ca2+ signals that were confined to PC distal dendrites. In the wild-type and mGluR1 mutant PCs, CF-induced Ca2+ signals involved both proximal and distal dendrites. Morphologically, CFs of GluRδ2 mutant mice extended to the superficial regions of the molecular layer, whereas those of wild-type and mGluR1 mutant mice did not innervate the superficial one-fifth of the molecular layer. It is therefore likely that surplus CFs of GluRδ2 mutant mice form ectopic synapses onto distal dendrites, whereas those of wild-type and mGluR1 mutant mice innervate proximal dendrites. These findings suggest that GluRδ2 is required for consolidating PF synapses and restricting CF synapses to the proximal dendrites, whereas the mGluR1-signaling pathway does not affect PF synaptogenesis but is involved in eliminating surplus CF synapses at the proximal dendrites.",
author = "Kouichi Hashimoto and Ryoichi Ichikawa and Hajime Takechi and Yoshiro Inoue and Atsu Aiba and Kenji Sakimura and Masayoshi Mishina and Tsutomu Hashikawa and Arthur Konnerth and Masahiko Watanabe and Masanobu Kano",
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Hashimoto, K, Ichikawa, R, Takechi, H, Inoue, Y, Aiba, A, Sakimura, K, Mishina, M, Hashikawa, T, Konnerth, A, Watanabe, M & Kano, M 2001, 'Roles of glutamate receptor δ2 subunit (GluRδ2) and metabotropic glutamate receptor subtype 1 (mGluR1) in climbing fiber synapse elimination during postnatal cerebellar development', Journal of Neuroscience, vol. 21, no. 24, pp. 9701-9712.

Roles of glutamate receptor δ2 subunit (GluRδ2) and metabotropic glutamate receptor subtype 1 (mGluR1) in climbing fiber synapse elimination during postnatal cerebellar development. / Hashimoto, Kouichi; Ichikawa, Ryoichi; Takechi, Hajime; Inoue, Yoshiro; Aiba, Atsu; Sakimura, Kenji; Mishina, Masayoshi; Hashikawa, Tsutomu; Konnerth, Arthur; Watanabe, Masahiko; Kano, Masanobu.

In: Journal of Neuroscience, Vol. 21, No. 24, 15.12.2001, p. 9701-9712.

Research output: Contribution to journalArticle

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T1 - Roles of glutamate receptor δ2 subunit (GluRδ2) and metabotropic glutamate receptor subtype 1 (mGluR1) in climbing fiber synapse elimination during postnatal cerebellar development

AU - Hashimoto, Kouichi

AU - Ichikawa, Ryoichi

AU - Takechi, Hajime

AU - Inoue, Yoshiro

AU - Aiba, Atsu

AU - Sakimura, Kenji

AU - Mishina, Masayoshi

AU - Hashikawa, Tsutomu

AU - Konnerth, Arthur

AU - Watanabe, Masahiko

AU - Kano, Masanobu

PY - 2001/12/15

Y1 - 2001/12/15

N2 - Climbing fiber (CF) synapse formation onto cerebellar Purkinje cells (PCs) is critically dependent on the synaptogenesis from parallel fibers (PFs), the other input to PCs. Previous studies revealed that deletion of the glutamate receptor δ2 subunit (GluRδ2) gene results in persistent multiple CF innervation of PCs with impaired PF synaptogenesis, whereas mutation of the metabotropic glutamate receptor subtype 1 (mGluR1) gene causes multiple CF innervation with normal PF synaptogenesis. We demonstrate that atypical CF-mediated EPSCs (CF-EPSCs) with slow rise times and small amplitudes coexisted with typical CF-EPSCs with fast rise times and large amplitudes in PCs from GluRδ2 mutant cerebellar slices. CF-EPSCs in mGluR1 mutant and wild-type PCs had fast rise times. Atypical slow CF responses of GluRδ2 mutant PCs were associated with voltage-dependent Ca2+ signals that were confined to PC distal dendrites. In the wild-type and mGluR1 mutant PCs, CF-induced Ca2+ signals involved both proximal and distal dendrites. Morphologically, CFs of GluRδ2 mutant mice extended to the superficial regions of the molecular layer, whereas those of wild-type and mGluR1 mutant mice did not innervate the superficial one-fifth of the molecular layer. It is therefore likely that surplus CFs of GluRδ2 mutant mice form ectopic synapses onto distal dendrites, whereas those of wild-type and mGluR1 mutant mice innervate proximal dendrites. These findings suggest that GluRδ2 is required for consolidating PF synapses and restricting CF synapses to the proximal dendrites, whereas the mGluR1-signaling pathway does not affect PF synaptogenesis but is involved in eliminating surplus CF synapses at the proximal dendrites.

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