ROR1 sustains caveolae and survival signalling as a scaffold of cavin-1 and caveolin-1

Tomoya Yamaguchi, Can Lu, Lisa Ida, Kiyoshi Yanagisawa, Jiro Usukura, Jinglei Cheng, Naoe Hotta, Yukako Shimada, Hisanori Isomura, Motoshi Suzuki, Toyoshi Fujimoto, Takashi Takahashi

Research output: Contribution to journalArticle

26 Citations (Scopus)

Abstract

The receptor tyrosine kinase-like orphan receptor 1 (ROR1) sustains prosurvival signalling directly downstream of the lineage-survival oncogene NKX2-1/TTF-1 in lung adenocarcinoma. Here we report an unanticipated function of this receptor tyrosine kinase (RTK) as a scaffold of cavin-1 and caveolin-1 (CAV1), two essential structural components of caveolae. This kinase-independent function of ROR1 facilitates the interactions of cavin-1 and CAV1 at the plasma membrane, thereby preventing the lysosomal degradation of CAV1. Caveolae structures and prosurvival signalling towards AKT through multiple RTKs are consequently sustained. These findings provide mechanistic insight into how ROR1 inhibition can overcome EGFR-tyrosine kinase inhibitor (TKI) resistance due to bypass signalling via diverse RTKs such as MET and IGF-IR, which is currently a major clinical obstacle. Considering its onco-embryonic expression, inhibition of the scaffold function of ROR1 in patients with lung adenocarcinoma is an attractive approach for improved treatment of this devastating cancer.

Original languageEnglish
Article number10060
JournalNature Communications
Volume7
DOIs
Publication statusPublished - 04-01-2016
Externally publishedYes

Fingerprint

Caveolin 1
Caveolae
Scaffolds
Receptor Tyrosine Kinase-like Orphan Receptors
Survival
tyrosine
Receptor Protein-Tyrosine Kinases
Cell membranes
Oncogenes
Protein-Tyrosine Kinases
lungs
Phosphotransferases
Cell Membrane
oncogenes
Degradation
bypasses
inhibitors
Caviner
Neoplasms
cancer

All Science Journal Classification (ASJC) codes

  • Chemistry(all)
  • Biochemistry, Genetics and Molecular Biology(all)
  • Physics and Astronomy(all)

Cite this

Yamaguchi, T., Lu, C., Ida, L., Yanagisawa, K., Usukura, J., Cheng, J., ... Takahashi, T. (2016). ROR1 sustains caveolae and survival signalling as a scaffold of cavin-1 and caveolin-1. Nature Communications, 7, [10060]. https://doi.org/10.1038/ncomms10060
Yamaguchi, Tomoya ; Lu, Can ; Ida, Lisa ; Yanagisawa, Kiyoshi ; Usukura, Jiro ; Cheng, Jinglei ; Hotta, Naoe ; Shimada, Yukako ; Isomura, Hisanori ; Suzuki, Motoshi ; Fujimoto, Toyoshi ; Takahashi, Takashi. / ROR1 sustains caveolae and survival signalling as a scaffold of cavin-1 and caveolin-1. In: Nature Communications. 2016 ; Vol. 7.
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abstract = "The receptor tyrosine kinase-like orphan receptor 1 (ROR1) sustains prosurvival signalling directly downstream of the lineage-survival oncogene NKX2-1/TTF-1 in lung adenocarcinoma. Here we report an unanticipated function of this receptor tyrosine kinase (RTK) as a scaffold of cavin-1 and caveolin-1 (CAV1), two essential structural components of caveolae. This kinase-independent function of ROR1 facilitates the interactions of cavin-1 and CAV1 at the plasma membrane, thereby preventing the lysosomal degradation of CAV1. Caveolae structures and prosurvival signalling towards AKT through multiple RTKs are consequently sustained. These findings provide mechanistic insight into how ROR1 inhibition can overcome EGFR-tyrosine kinase inhibitor (TKI) resistance due to bypass signalling via diverse RTKs such as MET and IGF-IR, which is currently a major clinical obstacle. Considering its onco-embryonic expression, inhibition of the scaffold function of ROR1 in patients with lung adenocarcinoma is an attractive approach for improved treatment of this devastating cancer.",
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Yamaguchi, T, Lu, C, Ida, L, Yanagisawa, K, Usukura, J, Cheng, J, Hotta, N, Shimada, Y, Isomura, H, Suzuki, M, Fujimoto, T & Takahashi, T 2016, 'ROR1 sustains caveolae and survival signalling as a scaffold of cavin-1 and caveolin-1', Nature Communications, vol. 7, 10060. https://doi.org/10.1038/ncomms10060

ROR1 sustains caveolae and survival signalling as a scaffold of cavin-1 and caveolin-1. / Yamaguchi, Tomoya; Lu, Can; Ida, Lisa; Yanagisawa, Kiyoshi; Usukura, Jiro; Cheng, Jinglei; Hotta, Naoe; Shimada, Yukako; Isomura, Hisanori; Suzuki, Motoshi; Fujimoto, Toyoshi; Takahashi, Takashi.

In: Nature Communications, Vol. 7, 10060, 04.01.2016.

Research output: Contribution to journalArticle

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AU - Hotta, Naoe

AU - Shimada, Yukako

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AU - Fujimoto, Toyoshi

AU - Takahashi, Takashi

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