Route of hepatocyte delivery affects hepatocyte engraftment in the spleen

Hideo Nagata, Masahiro Ito, Chiyoe Shirota, Albert Edge, Timothy C. McCowan, Ira J. Fox

Research output: Contribution to journalArticlepeer-review

51 Citations (Scopus)

Abstract

In laboratory animals, intrasplenic hepatocyte transplantation corrects the physiologic abnormalities associated with decompensated liver disease. The clinical experience with hepatocyte transplantation for cirrhosis has been disappointing when compared with laboratory experience. The route of hepatocyte delivery may influence hepatocyte engraftment and function. Outbred pigs were recipients of allogeneic pig hepatocytes. Donor hepatocytes were isolated by collagenase perfusion and labeled using 5(6)-carboxyfluorescein diacetate succinimidyl-ester (CMFSE). Cells were introduced into pig spleens by infusion through the splenic artery or by direct splenic puncture. Direct intrasplenic injection produced engraftment that was far superior to that obtained using splenic artery infusion. Splenic artery infusion produced a gastric erosion and large areas of splenic necrosis secondary to vascular occlusion with hepatocytes, whereas direct splenic injection was associated with clinically insignificant intraabdominal hemorrhage. The route of hepatocyte delivery may influence hepatocyte engraftment and explain the disparity in efficacy of hepatocyte transplantation between the laboratory and clinic.

Original languageEnglish
Pages (from-to)732-735
Number of pages4
JournalTransplantation
Volume76
Issue number4
DOIs
Publication statusPublished - 27-08-2003

All Science Journal Classification (ASJC) codes

  • Transplantation

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