RT1.P, rat class Ib genes related to mouse TL

Evidence that CD1 molecules but not authentic TL antigens are expressed by rat thymus

Akihiro Matsuura, Shinichi Takayama, Miyuki Kinebuchi, Yuji Hashimoto, Kiyoshi Kasai, Daisuke Kozutsumi, Shingo Ichimiya, Ryoichi Honda, Takashi Natori, Kokichi Kikuchi

Research output: Contribution to journalArticle

10 Citations (Scopus)

Abstract

CD1 and TL were once thought to be genetic homologues because of their thymus-specific expression. We investigated their equivalents in the rat to clarify whether their structure and pattern of expression are conserved in rodents. Two rat class Ib genes, containing 3' sequences very similar to mouse TL, were identified and designated RT1.P. Neither of them, however, can encode ordinary class I molecules due to the accumulation of harmful mutations in the 5' regions that are unique to RT1.P, while the 3' TL-like regions still retain protein-coding capacity. Comparison of the structural organization of three types of TL family genes, which include mouse T3/ T18-encoding TL antigens, mouse T1/T16, and rat RT1.P1/ P2 pseudogenes, revealed the presence of a clear demarcation between the type-specific and TL-specific sequences at intron 3. This finding suggests that recombination plays an important role in creating the TL family genes in rodents. Characteristic features of TL, such as a low level of polymorphism and linkage to the major histocompatibility complex, were also observed in the rat. On the other hand, rat CD1 molecules were expressed at a high level on the surface of thymocytes. Absence of authentic TL antigens and thymic expression of CD1d molecules in the rat suggest the plasticity and conservation of class Ib genes in rodent evolution. Functions of TL may be substituted with CD1 or other class Ib molecules expressed by rat thymus.

Original languageEnglish
Pages (from-to)293-306
Number of pages14
JournalImmunogenetics
Volume46
Issue number4
DOIs
Publication statusPublished - 13-08-1997

Fingerprint

Thymus Gland
Genes
Rodentia
CD1d Antigen
Pseudogenes
Thymocytes
thymus-leukemia antigens
Major Histocompatibility Complex
Introns
Genetic Recombination
Proteins

All Science Journal Classification (ASJC) codes

  • Immunology
  • Genetics

Cite this

Matsuura, Akihiro ; Takayama, Shinichi ; Kinebuchi, Miyuki ; Hashimoto, Yuji ; Kasai, Kiyoshi ; Kozutsumi, Daisuke ; Ichimiya, Shingo ; Honda, Ryoichi ; Natori, Takashi ; Kikuchi, Kokichi. / RT1.P, rat class Ib genes related to mouse TL : Evidence that CD1 molecules but not authentic TL antigens are expressed by rat thymus. In: Immunogenetics. 1997 ; Vol. 46, No. 4. pp. 293-306.
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abstract = "CD1 and TL were once thought to be genetic homologues because of their thymus-specific expression. We investigated their equivalents in the rat to clarify whether their structure and pattern of expression are conserved in rodents. Two rat class Ib genes, containing 3' sequences very similar to mouse TL, were identified and designated RT1.P. Neither of them, however, can encode ordinary class I molecules due to the accumulation of harmful mutations in the 5' regions that are unique to RT1.P, while the 3' TL-like regions still retain protein-coding capacity. Comparison of the structural organization of three types of TL family genes, which include mouse T3/ T18-encoding TL antigens, mouse T1/T16, and rat RT1.P1/ P2 pseudogenes, revealed the presence of a clear demarcation between the type-specific and TL-specific sequences at intron 3. This finding suggests that recombination plays an important role in creating the TL family genes in rodents. Characteristic features of TL, such as a low level of polymorphism and linkage to the major histocompatibility complex, were also observed in the rat. On the other hand, rat CD1 molecules were expressed at a high level on the surface of thymocytes. Absence of authentic TL antigens and thymic expression of CD1d molecules in the rat suggest the plasticity and conservation of class Ib genes in rodent evolution. Functions of TL may be substituted with CD1 or other class Ib molecules expressed by rat thymus.",
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Matsuura, A, Takayama, S, Kinebuchi, M, Hashimoto, Y, Kasai, K, Kozutsumi, D, Ichimiya, S, Honda, R, Natori, T & Kikuchi, K 1997, 'RT1.P, rat class Ib genes related to mouse TL: Evidence that CD1 molecules but not authentic TL antigens are expressed by rat thymus', Immunogenetics, vol. 46, no. 4, pp. 293-306. https://doi.org/10.1007/s002510050275

RT1.P, rat class Ib genes related to mouse TL : Evidence that CD1 molecules but not authentic TL antigens are expressed by rat thymus. / Matsuura, Akihiro; Takayama, Shinichi; Kinebuchi, Miyuki; Hashimoto, Yuji; Kasai, Kiyoshi; Kozutsumi, Daisuke; Ichimiya, Shingo; Honda, Ryoichi; Natori, Takashi; Kikuchi, Kokichi.

In: Immunogenetics, Vol. 46, No. 4, 13.08.1997, p. 293-306.

Research output: Contribution to journalArticle

TY - JOUR

T1 - RT1.P, rat class Ib genes related to mouse TL

T2 - Evidence that CD1 molecules but not authentic TL antigens are expressed by rat thymus

AU - Matsuura, Akihiro

AU - Takayama, Shinichi

AU - Kinebuchi, Miyuki

AU - Hashimoto, Yuji

AU - Kasai, Kiyoshi

AU - Kozutsumi, Daisuke

AU - Ichimiya, Shingo

AU - Honda, Ryoichi

AU - Natori, Takashi

AU - Kikuchi, Kokichi

PY - 1997/8/13

Y1 - 1997/8/13

N2 - CD1 and TL were once thought to be genetic homologues because of their thymus-specific expression. We investigated their equivalents in the rat to clarify whether their structure and pattern of expression are conserved in rodents. Two rat class Ib genes, containing 3' sequences very similar to mouse TL, were identified and designated RT1.P. Neither of them, however, can encode ordinary class I molecules due to the accumulation of harmful mutations in the 5' regions that are unique to RT1.P, while the 3' TL-like regions still retain protein-coding capacity. Comparison of the structural organization of three types of TL family genes, which include mouse T3/ T18-encoding TL antigens, mouse T1/T16, and rat RT1.P1/ P2 pseudogenes, revealed the presence of a clear demarcation between the type-specific and TL-specific sequences at intron 3. This finding suggests that recombination plays an important role in creating the TL family genes in rodents. Characteristic features of TL, such as a low level of polymorphism and linkage to the major histocompatibility complex, were also observed in the rat. On the other hand, rat CD1 molecules were expressed at a high level on the surface of thymocytes. Absence of authentic TL antigens and thymic expression of CD1d molecules in the rat suggest the plasticity and conservation of class Ib genes in rodent evolution. Functions of TL may be substituted with CD1 or other class Ib molecules expressed by rat thymus.

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