Salusin β is a surrogate ligand of the mas-like G protein-coupled receptor MrgA1

Zhiwei Wang, Toshio Takahashi, Yumiko Saito, Hiroshi Nagasaki, Nga Kim Ly, Hans Peter Nothacker, Rainer K. Reinscheid, Jun Yang, Jaw Kang Chang, Masayoshi Shichiri, Olivier Civelli

Research output: Contribution to journalArticlepeer-review

33 Citations (Scopus)


The mas-like G protein-coupled receptors form a subfamily of G protein-coupled receptors that includes variable member numbers across different species and that have been shown to bind a wide variety of ligands from peptides to amino acid derivatives. While screening a library of peptides against different orphan G protein-coupled receptors, we found that human salusin β activates the mouse mas-like G protein-coupled receptor, mMrgA1 with an EC50 of about 300 nM. Salusin β is a bioactive peptide recently discovered through bioinformatics analysis which stimulates arginine-vasopressin release from rat pituitary and causes rapid and profound hypotension and bradycardia. However, when we further analyzed the generality of the mMrgA1 activation, we found that human salusin β does not activate corresponding human mas-like G protein-coupled receptors. Our results show that human salusin β is a surrogate ligand of the mouse MrgA1 and raises a cautionary flag for experiments that analyze the pharmacological profiles of mas-like G protein-coupled receptors from different species.

Original languageEnglish
Pages (from-to)145-150
Number of pages6
JournalEuropean Journal of Pharmacology
Issue number3
Publication statusPublished - 13-06-2006
Externally publishedYes

All Science Journal Classification (ASJC) codes

  • Pharmacology


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