SAP155-mediated splicing of FUSE-binding protein-interacting repressor serves as a molecular switch for c-myc gene expression

Kazuyuki Matsushita, Toshiko Kajiwara, Mai Tamura, Mamoru Satoh, Nobuko Tanaka, Takeshi Tomonaga, Hisahiro Matsubara, Hideaki Shimada, Rei Yoshimoto, Akihiro Ito, Shuji Kubo, Tohru Natsume, David Levens, Minoru Yoshida, Fumio Nomura

Research output: Contribution to journalArticle

15 Citations (Scopus)

Abstract

The Far UpStream Element (FUSE)-binding protein-interacting repressor (FIR), a c-myc transcriptional suppressor, is alternatively spliced removing the transcriptional repression domain within exon 2 (FIRΔexon2) in colorectal cancers. SAP155 is a subunit of the essential splicing factor 3b (SF3b) subcomplex in the spliceosome. This study aims to study the significance of the FIR-SAP155 interaction for the coordination of c-myc transcription, pre-mRNA splicing, and c-Myc protein modification, as well as to interrogate FIRΔexon2 for other functions relating to altered FIR pre-mRNA splicing. Knockdown of SAP155 or FIR was used to investigate their reciprocal influence on each other and on c-myc transcription, pre-mRNA splicing, and protein expression. Pull down from HeLa cell nuclear extracts revealed the association of FIR, FIRΔexon2, and SF3b subunits. FIR and FIRΔexon2 were coimmunoprecipitated with SAP155. FIR and FIRΔexon2 adenovirus vector (Ad-FIR and Ad-FIRΔexon2, respectively) were prepared to test for their influence on c-myc expression. FIR, SAP155, SAP130, and c-myc were coordinately upregulated inhuman colorectal cancer. These results reveal that SAP155 and FIR/FIRΔexon2 forma complex and are mutually upregulating. Ad-FIRΔexon2 antagonized Ad-FIR transcriptional repression of c-myc in HeLa cells. Because FIRΔexon2 still carries RRM1 and RRM2 and binding activity to FUSE, it is able to displace repression competent FIR from FUSE in electrophoretic mobility shift assays, thus thwarting FIR-mediated transcriptional repression by FUSE. Thus aberrant FIRΔexon2 production in turn sustained c-Myc expression. In conclusion, altered FIR and c-myc pre-mRNA splicing, in addition to c-Myc expression by augmented FIR/FIRΔexon2- SAP155 complex, potentially contribute to colorectal cancer development.

Original languageEnglish
Pages (from-to)787-799
Number of pages13
JournalMolecular Cancer Research
Volume10
Issue number6
DOIs
Publication statusPublished - 01-06-2012

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myc Genes
RNA Precursors
Carrier Proteins
Gene Expression
Colorectal Neoplasms
HeLa Cells
Protein Splicing
Proto-Oncogene Proteins c-myc
Spliceosomes
Electrophoretic Mobility Shift Assay
Cell Extracts
Adenoviridae
Exons
RNA Splicing Factors

All Science Journal Classification (ASJC) codes

  • Molecular Biology
  • Oncology
  • Cancer Research

Cite this

Matsushita, Kazuyuki ; Kajiwara, Toshiko ; Tamura, Mai ; Satoh, Mamoru ; Tanaka, Nobuko ; Tomonaga, Takeshi ; Matsubara, Hisahiro ; Shimada, Hideaki ; Yoshimoto, Rei ; Ito, Akihiro ; Kubo, Shuji ; Natsume, Tohru ; Levens, David ; Yoshida, Minoru ; Nomura, Fumio. / SAP155-mediated splicing of FUSE-binding protein-interacting repressor serves as a molecular switch for c-myc gene expression. In: Molecular Cancer Research. 2012 ; Vol. 10, No. 6. pp. 787-799.
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abstract = "The Far UpStream Element (FUSE)-binding protein-interacting repressor (FIR), a c-myc transcriptional suppressor, is alternatively spliced removing the transcriptional repression domain within exon 2 (FIRΔexon2) in colorectal cancers. SAP155 is a subunit of the essential splicing factor 3b (SF3b) subcomplex in the spliceosome. This study aims to study the significance of the FIR-SAP155 interaction for the coordination of c-myc transcription, pre-mRNA splicing, and c-Myc protein modification, as well as to interrogate FIRΔexon2 for other functions relating to altered FIR pre-mRNA splicing. Knockdown of SAP155 or FIR was used to investigate their reciprocal influence on each other and on c-myc transcription, pre-mRNA splicing, and protein expression. Pull down from HeLa cell nuclear extracts revealed the association of FIR, FIRΔexon2, and SF3b subunits. FIR and FIRΔexon2 were coimmunoprecipitated with SAP155. FIR and FIRΔexon2 adenovirus vector (Ad-FIR and Ad-FIRΔexon2, respectively) were prepared to test for their influence on c-myc expression. FIR, SAP155, SAP130, and c-myc were coordinately upregulated inhuman colorectal cancer. These results reveal that SAP155 and FIR/FIRΔexon2 forma complex and are mutually upregulating. Ad-FIRΔexon2 antagonized Ad-FIR transcriptional repression of c-myc in HeLa cells. Because FIRΔexon2 still carries RRM1 and RRM2 and binding activity to FUSE, it is able to displace repression competent FIR from FUSE in electrophoretic mobility shift assays, thus thwarting FIR-mediated transcriptional repression by FUSE. Thus aberrant FIRΔexon2 production in turn sustained c-Myc expression. In conclusion, altered FIR and c-myc pre-mRNA splicing, in addition to c-Myc expression by augmented FIR/FIRΔexon2- SAP155 complex, potentially contribute to colorectal cancer development.",
author = "Kazuyuki Matsushita and Toshiko Kajiwara and Mai Tamura and Mamoru Satoh and Nobuko Tanaka and Takeshi Tomonaga and Hisahiro Matsubara and Hideaki Shimada and Rei Yoshimoto and Akihiro Ito and Shuji Kubo and Tohru Natsume and David Levens and Minoru Yoshida and Fumio Nomura",
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Matsushita, K, Kajiwara, T, Tamura, M, Satoh, M, Tanaka, N, Tomonaga, T, Matsubara, H, Shimada, H, Yoshimoto, R, Ito, A, Kubo, S, Natsume, T, Levens, D, Yoshida, M & Nomura, F 2012, 'SAP155-mediated splicing of FUSE-binding protein-interacting repressor serves as a molecular switch for c-myc gene expression', Molecular Cancer Research, vol. 10, no. 6, pp. 787-799. https://doi.org/10.1158/1541-7786.MCR-11-0462

SAP155-mediated splicing of FUSE-binding protein-interacting repressor serves as a molecular switch for c-myc gene expression. / Matsushita, Kazuyuki; Kajiwara, Toshiko; Tamura, Mai; Satoh, Mamoru; Tanaka, Nobuko; Tomonaga, Takeshi; Matsubara, Hisahiro; Shimada, Hideaki; Yoshimoto, Rei; Ito, Akihiro; Kubo, Shuji; Natsume, Tohru; Levens, David; Yoshida, Minoru; Nomura, Fumio.

In: Molecular Cancer Research, Vol. 10, No. 6, 01.06.2012, p. 787-799.

Research output: Contribution to journalArticle

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T1 - SAP155-mediated splicing of FUSE-binding protein-interacting repressor serves as a molecular switch for c-myc gene expression

AU - Matsushita, Kazuyuki

AU - Kajiwara, Toshiko

AU - Tamura, Mai

AU - Satoh, Mamoru

AU - Tanaka, Nobuko

AU - Tomonaga, Takeshi

AU - Matsubara, Hisahiro

AU - Shimada, Hideaki

AU - Yoshimoto, Rei

AU - Ito, Akihiro

AU - Kubo, Shuji

AU - Natsume, Tohru

AU - Levens, David

AU - Yoshida, Minoru

AU - Nomura, Fumio

PY - 2012/6/1

Y1 - 2012/6/1

N2 - The Far UpStream Element (FUSE)-binding protein-interacting repressor (FIR), a c-myc transcriptional suppressor, is alternatively spliced removing the transcriptional repression domain within exon 2 (FIRΔexon2) in colorectal cancers. SAP155 is a subunit of the essential splicing factor 3b (SF3b) subcomplex in the spliceosome. This study aims to study the significance of the FIR-SAP155 interaction for the coordination of c-myc transcription, pre-mRNA splicing, and c-Myc protein modification, as well as to interrogate FIRΔexon2 for other functions relating to altered FIR pre-mRNA splicing. Knockdown of SAP155 or FIR was used to investigate their reciprocal influence on each other and on c-myc transcription, pre-mRNA splicing, and protein expression. Pull down from HeLa cell nuclear extracts revealed the association of FIR, FIRΔexon2, and SF3b subunits. FIR and FIRΔexon2 were coimmunoprecipitated with SAP155. FIR and FIRΔexon2 adenovirus vector (Ad-FIR and Ad-FIRΔexon2, respectively) were prepared to test for their influence on c-myc expression. FIR, SAP155, SAP130, and c-myc were coordinately upregulated inhuman colorectal cancer. These results reveal that SAP155 and FIR/FIRΔexon2 forma complex and are mutually upregulating. Ad-FIRΔexon2 antagonized Ad-FIR transcriptional repression of c-myc in HeLa cells. Because FIRΔexon2 still carries RRM1 and RRM2 and binding activity to FUSE, it is able to displace repression competent FIR from FUSE in electrophoretic mobility shift assays, thus thwarting FIR-mediated transcriptional repression by FUSE. Thus aberrant FIRΔexon2 production in turn sustained c-Myc expression. In conclusion, altered FIR and c-myc pre-mRNA splicing, in addition to c-Myc expression by augmented FIR/FIRΔexon2- SAP155 complex, potentially contribute to colorectal cancer development.

AB - The Far UpStream Element (FUSE)-binding protein-interacting repressor (FIR), a c-myc transcriptional suppressor, is alternatively spliced removing the transcriptional repression domain within exon 2 (FIRΔexon2) in colorectal cancers. SAP155 is a subunit of the essential splicing factor 3b (SF3b) subcomplex in the spliceosome. This study aims to study the significance of the FIR-SAP155 interaction for the coordination of c-myc transcription, pre-mRNA splicing, and c-Myc protein modification, as well as to interrogate FIRΔexon2 for other functions relating to altered FIR pre-mRNA splicing. Knockdown of SAP155 or FIR was used to investigate their reciprocal influence on each other and on c-myc transcription, pre-mRNA splicing, and protein expression. Pull down from HeLa cell nuclear extracts revealed the association of FIR, FIRΔexon2, and SF3b subunits. FIR and FIRΔexon2 were coimmunoprecipitated with SAP155. FIR and FIRΔexon2 adenovirus vector (Ad-FIR and Ad-FIRΔexon2, respectively) were prepared to test for their influence on c-myc expression. FIR, SAP155, SAP130, and c-myc were coordinately upregulated inhuman colorectal cancer. These results reveal that SAP155 and FIR/FIRΔexon2 forma complex and are mutually upregulating. Ad-FIRΔexon2 antagonized Ad-FIR transcriptional repression of c-myc in HeLa cells. Because FIRΔexon2 still carries RRM1 and RRM2 and binding activity to FUSE, it is able to displace repression competent FIR from FUSE in electrophoretic mobility shift assays, thus thwarting FIR-mediated transcriptional repression by FUSE. Thus aberrant FIRΔexon2 production in turn sustained c-Myc expression. In conclusion, altered FIR and c-myc pre-mRNA splicing, in addition to c-Myc expression by augmented FIR/FIRΔexon2- SAP155 complex, potentially contribute to colorectal cancer development.

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