Screening of genes involved in chromosome segregation during meiosis I

In vitro gene transfer to mouse fetal oocytes

Makiko Tsutsumi, Hiroe Kowa-Sugiyama, Hasbaira Bolor, Hiroshi Kogo, Hidehito Inagaki, Tamae Oe, Koji Yamada, Mariko Ikeda, Tatsushi Toda, Hiroki Kurahashi

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

The events that take place during the prophase of meiosis I are essential for the correct segregation of homologous chromosomes. Defects in these processes likely contribute to infertility or recurrent pregnancy loss in humans. To screen for candidate genes for reproductive failure due to meiotic defects, we have analyzed the gene expression patterns in fetal, neonatal and adult gonads of both male and female mice by microarray and thereby identified 241 genes that are expressed specifically during prophase of meiosis I. Combined with our previous data obtained from developing spermatocytes, a total of 99 genes were identified that are upregulated in early prophase I. We confirmed the meiotic prophase I-specific expression of these genes using qRT-PCR. To further screen this panel for candidate genes that fulfill important roles in homologous pairing, synapsis and recombination, we established a gene transfer system for prophase I oocytes in combination with in vitro organ culture of ovaries, and successfully determined the localization of the selected genes. This gene set can thus serve as a resource for targeted sequence analysis via next-generation sequencing to identify the genes associated with human reproduction failure due to meiotic defects.

Original languageEnglish
Pages (from-to)515-522
Number of pages8
JournalJournal of Human Genetics
Volume57
Issue number8
DOIs
Publication statusPublished - 01-08-2012

Fingerprint

Chromosome Segregation
Meiosis
Oocytes
Meiotic Prophase I
Genes
Chromosome Pairing
Gene Expression
Spermatocytes
In Vitro Techniques
Organ Culture Techniques
Gonads
Infertility
Genetic Recombination
Reproduction
Sequence Analysis
Ovary
Pregnancy
Polymerase Chain Reaction

All Science Journal Classification (ASJC) codes

  • Genetics
  • Genetics(clinical)

Cite this

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title = "Screening of genes involved in chromosome segregation during meiosis I: In vitro gene transfer to mouse fetal oocytes",
abstract = "The events that take place during the prophase of meiosis I are essential for the correct segregation of homologous chromosomes. Defects in these processes likely contribute to infertility or recurrent pregnancy loss in humans. To screen for candidate genes for reproductive failure due to meiotic defects, we have analyzed the gene expression patterns in fetal, neonatal and adult gonads of both male and female mice by microarray and thereby identified 241 genes that are expressed specifically during prophase of meiosis I. Combined with our previous data obtained from developing spermatocytes, a total of 99 genes were identified that are upregulated in early prophase I. We confirmed the meiotic prophase I-specific expression of these genes using qRT-PCR. To further screen this panel for candidate genes that fulfill important roles in homologous pairing, synapsis and recombination, we established a gene transfer system for prophase I oocytes in combination with in vitro organ culture of ovaries, and successfully determined the localization of the selected genes. This gene set can thus serve as a resource for targeted sequence analysis via next-generation sequencing to identify the genes associated with human reproduction failure due to meiotic defects.",
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Screening of genes involved in chromosome segregation during meiosis I : In vitro gene transfer to mouse fetal oocytes. / Tsutsumi, Makiko; Kowa-Sugiyama, Hiroe; Bolor, Hasbaira; Kogo, Hiroshi; Inagaki, Hidehito; Oe, Tamae; Yamada, Koji; Ikeda, Mariko; Toda, Tatsushi; Kurahashi, Hiroki.

In: Journal of Human Genetics, Vol. 57, No. 8, 01.08.2012, p. 515-522.

Research output: Contribution to journalArticle

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AU - Tsutsumi, Makiko

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AU - Bolor, Hasbaira

AU - Kogo, Hiroshi

AU - Inagaki, Hidehito

AU - Oe, Tamae

AU - Yamada, Koji

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AU - Toda, Tatsushi

AU - Kurahashi, Hiroki

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