Secretory pathways and processing of human proopiomelanocortin (POMC) using transformed mouse cultured fibroblasts (L cells) and AtT20 cells by human POMC gene-Preembedding immunoelectron microscopic studies

R. Yoshiyuki Osamura; Yoji Suemizu; Shinichi Yoshimura; Sadaaki Hori; Kenichi Inada; Keiichi Watanabe; Yoshikatsu Nakai; Hiroo Imura

doi:10.1016/0196-9781(91)90092-4

Secretory pathways and processing of human proopiomelanocortin (POMC) using transformed mouse cultured fibroblasts (L cells) and AtT20 cells by human POMC gene-Preembedding immunoelectron microscopic studies

R. Yoshiyuki Osamura, Yoji Suemizu, Shinichi Yoshimura, Sadaaki Hori, Kenichi Inada, Keiichi Watanabe, Yoshikatsu Nakai, Hiroo Imura

Research output: Contribution to journal › Article › peer-review

8 Citations (Scopus)

Abstract

In order to elucidate the relationship between secretory pathway and processing for precursor molecule of peptide hormones, we performed immunoelectron microscopic studies to localize POMC-derived peptides in mouse cultured L cells (fibroblasts without secretory granules) and in mouse AtT20 cells (ACTH secreting pituitary tumor cells with secretory granules) which had been transformed with human POMC gene. From the electron microscopic localization patterns, L42 cells were considered to serve as a model of constitutive pathway without processing of POMC, and A53 cells were considered to serve as a model of transgranular (regulated) pathway with processing of POMC. Immunoblotting supported these interpretations.

Original language	English
Pages (from-to)	503-507
Number of pages	5
Journal	Peptides
Volume	12
Issue number	3
DOIs	https://doi.org/10.1016/0196-9781(91)90092-4
Publication status	Published - 1991
Externally published	Yes

All Science Journal Classification (ASJC) codes

Biochemistry
Physiology
Endocrinology
Cellular and Molecular Neuroscience

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Osamura, R. Y., Suemizu, Y., Yoshimura, S., Hori, S., Inada, K., Watanabe, K., Nakai, Y., & Imura, H. (1991). Secretory pathways and processing of human proopiomelanocortin (POMC) using transformed mouse cultured fibroblasts (L cells) and AtT20 cells by human POMC gene-Preembedding immunoelectron microscopic studies. Peptides, 12(3), 503-507. https://doi.org/10.1016/0196-9781(91)90092-4

@article{fb4026ca0088457cbc05789f903373a4,

title = "Secretory pathways and processing of human proopiomelanocortin (POMC) using transformed mouse cultured fibroblasts (L cells) and AtT20 cells by human POMC gene-Preembedding immunoelectron microscopic studies",

abstract = "In order to elucidate the relationship between secretory pathway and processing for precursor molecule of peptide hormones, we performed immunoelectron microscopic studies to localize POMC-derived peptides in mouse cultured L cells (fibroblasts without secretory granules) and in mouse AtT20 cells (ACTH secreting pituitary tumor cells with secretory granules) which had been transformed with human POMC gene. From the electron microscopic localization patterns, L42 cells were considered to serve as a model of constitutive pathway without processing of POMC, and A53 cells were considered to serve as a model of transgranular (regulated) pathway with processing of POMC. Immunoblotting supported these interpretations.",

author = "Osamura, {R. Yoshiyuki} and Yoji Suemizu and Shinichi Yoshimura and Sadaaki Hori and Kenichi Inada and Keiichi Watanabe and Yoshikatsu Nakai and Hiroo Imura",

year = "1991",

doi = "10.1016/0196-9781(91)90092-4",

language = "English",

volume = "12",

pages = "503--507",

journal = "Peptides",

issn = "0196-9781",

publisher = "Elsevier Inc.",

number = "3",

}

Osamura, RY, Suemizu, Y, Yoshimura, S, Hori, S, Inada, K, Watanabe, K, Nakai, Y & Imura, H 1991, 'Secretory pathways and processing of human proopiomelanocortin (POMC) using transformed mouse cultured fibroblasts (L cells) and AtT20 cells by human POMC gene-Preembedding immunoelectron microscopic studies', Peptides, vol. 12, no. 3, pp. 503-507. https://doi.org/10.1016/0196-9781(91)90092-4

Secretory pathways and processing of human proopiomelanocortin (POMC) using transformed mouse cultured fibroblasts (L cells) and AtT20 cells by human POMC gene-Preembedding immunoelectron microscopic studies. / Osamura, R. Yoshiyuki; Suemizu, Yoji; Yoshimura, Shinichi et al.
In: Peptides, Vol. 12, No. 3, 1991, p. 503-507.

Research output: Contribution to journal › Article › peer-review

TY - JOUR

T1 - Secretory pathways and processing of human proopiomelanocortin (POMC) using transformed mouse cultured fibroblasts (L cells) and AtT20 cells by human POMC gene-Preembedding immunoelectron microscopic studies

AU - Osamura, R. Yoshiyuki

AU - Suemizu, Yoji

AU - Yoshimura, Shinichi

AU - Hori, Sadaaki

AU - Inada, Kenichi

AU - Watanabe, Keiichi

AU - Nakai, Yoshikatsu

AU - Imura, Hiroo

PY - 1991

Y1 - 1991

N2 - In order to elucidate the relationship between secretory pathway and processing for precursor molecule of peptide hormones, we performed immunoelectron microscopic studies to localize POMC-derived peptides in mouse cultured L cells (fibroblasts without secretory granules) and in mouse AtT20 cells (ACTH secreting pituitary tumor cells with secretory granules) which had been transformed with human POMC gene. From the electron microscopic localization patterns, L42 cells were considered to serve as a model of constitutive pathway without processing of POMC, and A53 cells were considered to serve as a model of transgranular (regulated) pathway with processing of POMC. Immunoblotting supported these interpretations.

AB - In order to elucidate the relationship between secretory pathway and processing for precursor molecule of peptide hormones, we performed immunoelectron microscopic studies to localize POMC-derived peptides in mouse cultured L cells (fibroblasts without secretory granules) and in mouse AtT20 cells (ACTH secreting pituitary tumor cells with secretory granules) which had been transformed with human POMC gene. From the electron microscopic localization patterns, L42 cells were considered to serve as a model of constitutive pathway without processing of POMC, and A53 cells were considered to serve as a model of transgranular (regulated) pathway with processing of POMC. Immunoblotting supported these interpretations.

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DO - 10.1016/0196-9781(91)90092-4

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JO - Peptides

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Secretory pathways and processing of human proopiomelanocortin (POMC) using transformed mouse cultured fibroblasts (L cells) and AtT20 cells by human POMC gene-Preembedding immunoelectron microscopic studies

Abstract

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