Selective inhibition of DNA polymerase ε by phosphatidylinositol

Mami Shoji-kawaguchi, Shunji Izuta, Keiko Tamiya-koizumi, Motoshi Suzuki, Shonen Yoshida

Research output: Contribution to journalArticle

32 Citations (Scopus)

Abstract

We studied the effects of various phoapholipids on the DNA synthesizing reactions by calf thymus DNA polymerases α, δ, and ε. Of these three enzymes, DNA polymerase ε was most sensitive to acidic phospholipids, i.e., phosphatidylinositol (PI), phosphatidylinositol 4-monophosphate (PIP), phosphatidylserine (PS), phosphatidic acid (PA), and cardiolipin (CAR). Of these acidic phospholipids, PI (from bovine liver) is of special interest because it inhibited DNA polymerase ε much more strongly than DNA polymerase α and δ. The inhibition of DNA polymerase ε by PI was competitive with the DNA template-primer and was noncompetitive with dTTP substrate. The K1 value was estimated to be 16 μM. These results indicate that PI from bovine liver can be used as a specific inhibitor for DNA polymerase ε to analyze its role in DNA replication. Interestingly, the PI isolated from soybean, which has a different fatty acid composition, inhibited not only DNA polymerase ε but also DNA polymerase α.

Original languageEnglish
Pages (from-to)1095-1099
Number of pages5
JournalJournal of Biochemistry
Volume117
Issue number5
DOIs
Publication statusPublished - 01-01-1995
Externally publishedYes

Fingerprint

DNA-Directed DNA Polymerase
Phosphatidylinositols
Liver
DNA
Phospholipids
Nucleic Acid Synthesis Inhibitors
Phosphatidic Acids
Cardiolipins
DNA Primers
Phosphatidylserines
DNA Replication
Soybeans
Fatty Acids
Substrates
Enzymes
Chemical analysis

All Science Journal Classification (ASJC) codes

  • Biochemistry
  • Molecular Biology

Cite this

Shoji-kawaguchi, Mami ; Izuta, Shunji ; Tamiya-koizumi, Keiko ; Suzuki, Motoshi ; Yoshida, Shonen. / Selective inhibition of DNA polymerase ε by phosphatidylinositol. In: Journal of Biochemistry. 1995 ; Vol. 117, No. 5. pp. 1095-1099.
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Shoji-kawaguchi, M, Izuta, S, Tamiya-koizumi, K, Suzuki, M & Yoshida, S 1995, 'Selective inhibition of DNA polymerase ε by phosphatidylinositol', Journal of Biochemistry, vol. 117, no. 5, pp. 1095-1099. https://doi.org/10.1093/oxfordjournals.jbchem.a124812

Selective inhibition of DNA polymerase ε by phosphatidylinositol. / Shoji-kawaguchi, Mami; Izuta, Shunji; Tamiya-koizumi, Keiko; Suzuki, Motoshi; Yoshida, Shonen.

In: Journal of Biochemistry, Vol. 117, No. 5, 01.01.1995, p. 1095-1099.

Research output: Contribution to journalArticle

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