Selective inhibition of DNA polymerase ε by phosphatidylinositol

Mami Shoji-kawaguchi, Shunji Izuta, Keiko Tamiya-koizumi, Motoshi Suzuki, Shonen Yoshida

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32 Citations (Scopus)

Abstract

We studied the effects of various phoapholipids on the DNA synthesizing reactions by calf thymus DNA polymerases α, δ, and ε. Of these three enzymes, DNA polymerase ε was most sensitive to acidic phospholipids, i.e., phosphatidylinositol (PI), phosphatidylinositol 4-monophosphate (PIP), phosphatidylserine (PS), phosphatidic acid (PA), and cardiolipin (CAR). Of these acidic phospholipids, PI (from bovine liver) is of special interest because it inhibited DNA polymerase ε much more strongly than DNA polymerase α and δ. The inhibition of DNA polymerase ε by PI was competitive with the DNA template-primer and was noncompetitive with dTTP substrate. The K1 value was estimated to be 16 μM. These results indicate that PI from bovine liver can be used as a specific inhibitor for DNA polymerase ε to analyze its role in DNA replication. Interestingly, the PI isolated from soybean, which has a different fatty acid composition, inhibited not only DNA polymerase ε but also DNA polymerase α.

Original languageEnglish
Pages (from-to)1095-1099
Number of pages5
JournalJournal of Biochemistry
Volume117
Issue number5
DOIs
Publication statusPublished - 05-1995
Externally publishedYes

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All Science Journal Classification (ASJC) codes

  • Biochemistry
  • Molecular Biology

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