Selective staining of hepatitis b surface antigen in thick epoxy sections of liver

Mikihiro Shamoto, Haruko Nishio, Yoshimitsu Katoh, Hideo Senba, Madoka Ito

Research output: Contribution to journalArticlepeer-review

Abstract

Hepatitis B surface antigen (HB2Ag) in epoxy-embedded liver tissues can be stained by aldehyde-fuchsin stain. Sections are oxidized in KMnO4 acidified with H2SO4, then bleached in NaHSO3, both at 70 C. Heating for oxidation and bleaching are absolutely necessary. Diluted aldehyde-fuchsin stain adjusted to pH 1.5 to 1.8 with NaOH is used for staining. HBsAg is specifically stained purple. Other components such as mitochondria and bile pigments are also stained, but are easily distinguished from HBsAg. This staining method is advantageous for the identification of HBsAg-positive cells for electron microscopic observation.

Original languageEnglish
Pages (from-to)285-289
Number of pages5
JournalBiotechnic and Histochemistry
Volume52
Issue number5
DOIs
Publication statusPublished - 1977

All Science Journal Classification (ASJC) codes

  • Histology
  • Medical Laboratory Technology

Fingerprint

Dive into the research topics of 'Selective staining of hepatitis b surface antigen in thick epoxy sections of liver'. Together they form a unique fingerprint.

Cite this