TY - JOUR
T1 - Selective staining of hepatitis b surface antigen in thick epoxy sections of liver
AU - Shamoto, Mikihiro
AU - Nishio, Haruko
AU - Katoh, Yoshimitsu
AU - Senba, Hideo
AU - Ito, Madoka
PY - 1977
Y1 - 1977
N2 - Hepatitis B surface antigen (HB2Ag) in epoxy-embedded liver tissues can be stained by aldehyde-fuchsin stain. Sections are oxidized in KMnO4 acidified with H2SO4, then bleached in NaHSO3, both at 70 C. Heating for oxidation and bleaching are absolutely necessary. Diluted aldehyde-fuchsin stain adjusted to pH 1.5 to 1.8 with NaOH is used for staining. HBsAg is specifically stained purple. Other components such as mitochondria and bile pigments are also stained, but are easily distinguished from HBsAg. This staining method is advantageous for the identification of HBsAg-positive cells for electron microscopic observation.
AB - Hepatitis B surface antigen (HB2Ag) in epoxy-embedded liver tissues can be stained by aldehyde-fuchsin stain. Sections are oxidized in KMnO4 acidified with H2SO4, then bleached in NaHSO3, both at 70 C. Heating for oxidation and bleaching are absolutely necessary. Diluted aldehyde-fuchsin stain adjusted to pH 1.5 to 1.8 with NaOH is used for staining. HBsAg is specifically stained purple. Other components such as mitochondria and bile pigments are also stained, but are easily distinguished from HBsAg. This staining method is advantageous for the identification of HBsAg-positive cells for electron microscopic observation.
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U2 - 10.3109/10520297709116795
DO - 10.3109/10520297709116795
M3 - Article
AN - SCOPUS:84907132107
VL - 52
SP - 285
EP - 289
JO - Biotechnic and Histochemistry
JF - Biotechnic and Histochemistry
SN - 1052-0295
IS - 5
ER -