Selective staining of hepatitis b surface antigen in thick epoxy sections of liver

Mikihiro Shamoto; Haruko Nishio; Yoshimitsu Katoh; Hideo Senba; Madoka Ito

doi:10.3109/10520297709116795

Selective staining of hepatitis b surface antigen in thick epoxy sections of liver

Mikihiro Shamoto
, Haruko Nishio
, Yoshimitsu Katoh
, Hideo Senba
, Madoka Ito

Cadaver Surgical Training Center

Research output: Contribution to journal › Article › peer-review

Abstract

Hepatitis B surface antigen (HB₂Ag) in epoxy-embedded liver tissues can be stained by aldehyde-fuchsin stain. Sections are oxidized in KMnO₄ acidified with H₂SO₄, then bleached in NaHSO₃, both at 70 C. Heating for oxidation and bleaching are absolutely necessary. Diluted aldehyde-fuchsin stain adjusted to pH 1.5 to 1.8 with NaOH is used for staining. HB_sAg is specifically stained purple. Other components such as mitochondria and bile pigments are also stained, but are easily distinguished from HB_sAg. This staining method is advantageous for the identification of HB_sAg-positive cells for electron microscopic observation.

Original language	English
Pages (from-to)	285-289
Number of pages	5
Journal	Biotechnic and Histochemistry
Volume	52
Issue number	5
DOIs	https://doi.org/10.3109/10520297709116795
Publication status	Published - 1977

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This output contributes to the following UN Sustainable Development Goals (SDGs)

SDG 3 Good Health and Well-being

All Science Journal Classification (ASJC) codes

Histology
Medical Laboratory Technology

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10.3109/10520297709116795

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title = "Selective staining of hepatitis b surface antigen in thick epoxy sections of liver",

abstract = "Hepatitis B surface antigen (HB2Ag) in epoxy-embedded liver tissues can be stained by aldehyde-fuchsin stain. Sections are oxidized in KMnO4 acidified with H2SO4, then bleached in NaHSO3, both at 70 C. Heating for oxidation and bleaching are absolutely necessary. Diluted aldehyde-fuchsin stain adjusted to pH 1.5 to 1.8 with NaOH is used for staining. HBsAg is specifically stained purple. Other components such as mitochondria and bile pigments are also stained, but are easily distinguished from HBsAg. This staining method is advantageous for the identification of HBsAg-positive cells for electron microscopic observation.",

author = "Mikihiro Shamoto and Haruko Nishio and Yoshimitsu Katoh and Hideo Senba and Madoka Ito",

year = "1977",

doi = "10.3109/10520297709116795",

language = "English",

volume = "52",

pages = "285--289",

journal = "Biotechnic and Histochemistry",

issn = "1052-0295",

publisher = "Taylor and Francis Ltd.",

number = "5",

}

TY - JOUR

T1 - Selective staining of hepatitis b surface antigen in thick epoxy sections of liver

AU - Shamoto, Mikihiro

AU - Nishio, Haruko

AU - Katoh, Yoshimitsu

AU - Senba, Hideo

AU - Ito, Madoka

PY - 1977

Y1 - 1977

N2 - Hepatitis B surface antigen (HB2Ag) in epoxy-embedded liver tissues can be stained by aldehyde-fuchsin stain. Sections are oxidized in KMnO4 acidified with H2SO4, then bleached in NaHSO3, both at 70 C. Heating for oxidation and bleaching are absolutely necessary. Diluted aldehyde-fuchsin stain adjusted to pH 1.5 to 1.8 with NaOH is used for staining. HBsAg is specifically stained purple. Other components such as mitochondria and bile pigments are also stained, but are easily distinguished from HBsAg. This staining method is advantageous for the identification of HBsAg-positive cells for electron microscopic observation.

AB - Hepatitis B surface antigen (HB2Ag) in epoxy-embedded liver tissues can be stained by aldehyde-fuchsin stain. Sections are oxidized in KMnO4 acidified with H2SO4, then bleached in NaHSO3, both at 70 C. Heating for oxidation and bleaching are absolutely necessary. Diluted aldehyde-fuchsin stain adjusted to pH 1.5 to 1.8 with NaOH is used for staining. HBsAg is specifically stained purple. Other components such as mitochondria and bile pigments are also stained, but are easily distinguished from HBsAg. This staining method is advantageous for the identification of HBsAg-positive cells for electron microscopic observation.

UR - https://www.scopus.com/pages/publications/84907132107

UR - https://www.scopus.com/pages/publications/84907132107#tab=citedBy

U2 - 10.3109/10520297709116795

DO - 10.3109/10520297709116795

M3 - Article

AN - SCOPUS:84907132107

SN - 1052-0295

VL - 52

SP - 285

EP - 289

JO - Biotechnic and Histochemistry

JF - Biotechnic and Histochemistry

IS - 5

ER -

Selective staining of hepatitis b surface antigen in thick epoxy sections of liver

Abstract

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