Selegiline potentiates the effects of EGb 761 in response to ischemic brain injury

Y. S. Kwon, H. S. Ann, Toshitaka Nabeshima, E. J. Shin, W. K. Kim, J. H. Jhoo, W. K. Jhoo, M. B. Wie, Y. S. Kim, K. J. Jang, H. C. Kim

Research output: Contribution to journalArticle

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Abstract

We evaluated whether combined treatment with selegiline, a selective MAO-B inhibitor, and EGb 761, a standard extract of Ginkgo biloba, has synergistic effects against ischemic reperfusion injury (IRI) in gerbils. Interestingly, we observed that pretreatment with EGb 761 significantly attenuated selegiline-induced hyperactivity. This finding paralleled striatal fos-related antigen immunoreactivity (FRA-IR) in mice. Four minutes of bilateral carotid artery occlusion caused substantial cell loss in the CA1 of the hippocampus 5 days post-ischemic insult. Pretreatment with EGb 761, with or without selegiline, significantly attenuated this neuronal loss. Combined treatment with EGb 761 plus selegiline was more efficacious in preventing this loss. Synaptosomal formations of protein carbonyl, lipid peroxidation (malondialdehyde (MDA) + 4-hydroxyalkenal (4-HDA)), and reactive oxygen species (ROS) in the hippocampus remained elevated 5 days post-ischemic insult. The antioxidant effects appeared to be most significant in the group treated with EGb 761 plus selegiline. This combined treatment produced more significant attenuation of IRI-induced alterations in intramitochondrial calcium accumulation, the mitochondrial transmembrane potential, and mitochondrial Mn-superoxide dismutase-like immunoreactivity (Mn-SOD-IR) than either treatment alone. Our results suggest that co-administration of EGb 761 and selegiline produces significant neuroprotective effects via suppression of oxidative stress and mitochondrial dysfunction without affecting neurological function.

Original languageEnglish
Pages (from-to)157-170
Number of pages14
JournalNeurochemistry International
Volume45
Issue number1
DOIs
Publication statusPublished - 01-07-2004
Externally publishedYes

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Selegiline
Brain Injuries
Reperfusion Injury
Hippocampus
Protein Carbonylation
Proto-Oncogene Proteins c-fos
Corpus Striatum
Ginkgo biloba
Monoamine Oxidase Inhibitors
Gerbillinae
Monoamine Oxidase
Neuroprotective Agents
Malondialdehyde
Carotid Arteries
Membrane Potentials
Lipid Peroxidation
Superoxide Dismutase
Ginkgo biloba extract 761
Reactive Oxygen Species
Oxidative Stress

All Science Journal Classification (ASJC) codes

  • Cellular and Molecular Neuroscience
  • Cell Biology

Cite this

Kwon, Y. S. ; Ann, H. S. ; Nabeshima, Toshitaka ; Shin, E. J. ; Kim, W. K. ; Jhoo, J. H. ; Jhoo, W. K. ; Wie, M. B. ; Kim, Y. S. ; Jang, K. J. ; Kim, H. C. / Selegiline potentiates the effects of EGb 761 in response to ischemic brain injury. In: Neurochemistry International. 2004 ; Vol. 45, No. 1. pp. 157-170.
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Kwon, YS, Ann, HS, Nabeshima, T, Shin, EJ, Kim, WK, Jhoo, JH, Jhoo, WK, Wie, MB, Kim, YS, Jang, KJ & Kim, HC 2004, 'Selegiline potentiates the effects of EGb 761 in response to ischemic brain injury', Neurochemistry International, vol. 45, no. 1, pp. 157-170. https://doi.org/10.1016/j.neuint.2003.10.005

Selegiline potentiates the effects of EGb 761 in response to ischemic brain injury. / Kwon, Y. S.; Ann, H. S.; Nabeshima, Toshitaka; Shin, E. J.; Kim, W. K.; Jhoo, J. H.; Jhoo, W. K.; Wie, M. B.; Kim, Y. S.; Jang, K. J.; Kim, H. C.

In: Neurochemistry International, Vol. 45, No. 1, 01.07.2004, p. 157-170.

Research output: Contribution to journalArticle

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T1 - Selegiline potentiates the effects of EGb 761 in response to ischemic brain injury

AU - Kwon, Y. S.

AU - Ann, H. S.

AU - Nabeshima, Toshitaka

AU - Shin, E. J.

AU - Kim, W. K.

AU - Jhoo, J. H.

AU - Jhoo, W. K.

AU - Wie, M. B.

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AU - Jang, K. J.

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