Abstract
Previous studies have reported that a strong correlation between the estimated cumulative thermal exposure in the crystalline lens and the incidence of nuclear cataracts; however, the precise relationship between temperature and cataracts remains to be fully elucidated. In the present study, the shotgun liquid chromatography/mass spectroscopy-based global proteomic approach was applied to investigate cataract-inducing factors in lens cultured at normal (35.0°C) and slightly warmer (37.5°C) conditions. In the rat lens, 190 proteins (total) were identified. Of these, 48 proteins (25.3%) were found in lenses cultured at both 35.0°C and 37.5°C. Moreover, 85 proteins (44.7%) were unique to lenses cultured at 35.0°C, while 57 proteins (30.0%) were unique to lenses cultured at 37.5°C. Protein expression changes in rat lenses cultured at 37.5°C were examined using a label-free semiquantitative approach that uses spectral counting and Gene Ontology analysis. Filensin and vimentin protein expression, key factors in maintaining lens structure, were decreased. These findings may serve as a valuable indicator for elucidating the relationship between temperature and the onset of nuclear cataracts.
Original language | English |
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Article number | 26 |
Journal | Molecular Medicine Reports |
Volume | 31 |
Issue number | 1 |
DOIs | |
Publication status | Published - 01-2025 |
All Science Journal Classification (ASJC) codes
- Biochemistry
- Molecular Medicine
- Molecular Biology
- Genetics
- Oncology
- Cancer Research