Semiquantitative analysis of protein expression in heated rat lens using shotgun proteomics

Hiroko Otake, Shuya Masuda, Tetsushi Yamamoto, Yoshiki Miyata, Yosuke Nakazawa, Naoki Yamamoto, Atsushi Taga, Hiroshi Sasaki, Noriaki Nagai

Research output: Contribution to journalArticlepeer-review

Abstract

Previous studies have reported that a strong correlation between the estimated cumulative thermal exposure in the crystalline lens and the incidence of nuclear cataracts; however, the precise relationship between temperature and cataracts remains to be fully elucidated. In the present study, the shotgun liquid chromatography/mass spectroscopy-based global proteomic approach was applied to investigate cataract-inducing factors in lens cultured at normal (35.0°C) and slightly warmer (37.5°C) conditions. In the rat lens, 190 proteins (total) were identified. Of these, 48 proteins (25.3%) were found in lenses cultured at both 35.0°C and 37.5°C. Moreover, 85 proteins (44.7%) were unique to lenses cultured at 35.0°C, while 57 proteins (30.0%) were unique to lenses cultured at 37.5°C. Protein expression changes in rat lenses cultured at 37.5°C were examined using a label-free semiquantitative approach that uses spectral counting and Gene Ontology analysis. Filensin and vimentin protein expression, key factors in maintaining lens structure, were decreased. These findings may serve as a valuable indicator for elucidating the relationship between temperature and the onset of nuclear cataracts.

Original languageEnglish
Article number26
JournalMolecular Medicine Reports
Volume31
Issue number1
DOIs
Publication statusPublished - 01-2025

All Science Journal Classification (ASJC) codes

  • Biochemistry
  • Molecular Medicine
  • Molecular Biology
  • Genetics
  • Oncology
  • Cancer Research

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