TY - JOUR
T1 - Serial analysis of gene expression in HIV-1-infected T cell lines
AU - Ryo, Akihide
AU - Suzuki, Youichi
AU - Ichiyama, Kouji
AU - Wakatsuki, Toru
AU - Kondoh, Nobuo
AU - Hada, Akiyuki
AU - Yamamoto, Mikio
AU - Yamamoto, Naoki
N1 - Funding Information:
The authors thank Masaaki Arai, Kenji Tanaka, Masahiro Shuda and Mizue Shichita for technical assistance. We would like to acknowledge the Human Genome Center of the Institute of Medical Science, The University of Tokyo for providing valuable databases. A.R. is a fellow of the Japan Society for the Promotion of Science. This work was supported by grants from Health Sciences of Organization for Drug ADR Relief, R&D Promotion and Product Review of Japan and CREST (Core Research for Evolutional Science and Technology) of Japan Science and Technology Corporation (JST).
PY - 1999/11/26
Y1 - 1999/11/26
N2 - The gene expression profile of the HIV-1 infection state was analyzed in the human T cell line MOLT-4. Using the serial analysis of gene expression (SAGE) method, a total of 142 603 SAGE tags were sequenced and identified, representing 43 581 unique mRNA species. Comparison of expression patterns revealed that 53 cellular genes were differentially expressed upon HIV-1 infection. Northern blot and RT-PCR analyses confirmed the altered expression of the genes in both MOLT-4 and MT-4 cells. Up-regulated genes were mainly composed of transcription factors and genes related to T cell activation, whereas down-regulated genes were comprised of mitochondrial proteins, actin- related factors and translational factors. These findings indicate that persistent T cell activation, which may accelerate HIV-1 replication, and the disruption of cellular housekeeping genes including those involved in anti- apoptotic systems, may play an important role in HIV-1-induced pathogenesis.
AB - The gene expression profile of the HIV-1 infection state was analyzed in the human T cell line MOLT-4. Using the serial analysis of gene expression (SAGE) method, a total of 142 603 SAGE tags were sequenced and identified, representing 43 581 unique mRNA species. Comparison of expression patterns revealed that 53 cellular genes were differentially expressed upon HIV-1 infection. Northern blot and RT-PCR analyses confirmed the altered expression of the genes in both MOLT-4 and MT-4 cells. Up-regulated genes were mainly composed of transcription factors and genes related to T cell activation, whereas down-regulated genes were comprised of mitochondrial proteins, actin- related factors and translational factors. These findings indicate that persistent T cell activation, which may accelerate HIV-1 replication, and the disruption of cellular housekeeping genes including those involved in anti- apoptotic systems, may play an important role in HIV-1-induced pathogenesis.
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U2 - 10.1016/S0014-5793(99)01526-4
DO - 10.1016/S0014-5793(99)01526-4
M3 - Article
C2 - 10580116
AN - SCOPUS:0032714190
SN - 0014-5793
VL - 462
SP - 182
EP - 186
JO - FEBS Letters
JF - FEBS Letters
IS - 1-2
ER -
