TY - JOUR
T1 - Sero- and molecular typing of Duffy blood group in Southeast Asians and Oceanians
AU - Shimizu, Yuko
AU - Ao, Hiroko
AU - Soemantri, Augustinus
AU - Tiwawech, Danai
AU - Settheetham-Ishida, Wannapa
AU - Kayame, Oktovianus Wellem
AU - Kimura, Masako
AU - Nishioka, Tomoki
AU - Ishida, Takafumi
PY - 2000/6
Y1 - 2000/6
N2 - Duffy blood group was studied among malaria-endemic Thai and Indonesian populations: Hmong (n = 103), Akha (n = 218), Lisu (n = 44), Bugis (n = 95), Toraja (n = 77), Dani (n = 44), Mee (n = 80) and Irianese (n = 81). Phenotypes were studied by the ordinal indirect Coombs' test and genotypes were determined by the polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) test. DNA analysis was used to type the Duffy blood group system. Deduced allele frequencies of Fya (0.958 ~ 1.0) based on the phenotypes were similar to those in other populations in Southeast Asia and Oceania. The study revealed the presence of Fya-antigens showing weak reactivity to antisera as well as a discrepancy between the genotype shown by the PCR-RFLP study and that predicted by the phenotype. The PCR-RFLP study also suggested the presence of an alternative genetic basis for the Fy(a - b -) phenotype, which differs from the African type.
AB - Duffy blood group was studied among malaria-endemic Thai and Indonesian populations: Hmong (n = 103), Akha (n = 218), Lisu (n = 44), Bugis (n = 95), Toraja (n = 77), Dani (n = 44), Mee (n = 80) and Irianese (n = 81). Phenotypes were studied by the ordinal indirect Coombs' test and genotypes were determined by the polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) test. DNA analysis was used to type the Duffy blood group system. Deduced allele frequencies of Fya (0.958 ~ 1.0) based on the phenotypes were similar to those in other populations in Southeast Asia and Oceania. The study revealed the presence of Fya-antigens showing weak reactivity to antisera as well as a discrepancy between the genotype shown by the PCR-RFLP study and that predicted by the phenotype. The PCR-RFLP study also suggested the presence of an alternative genetic basis for the Fy(a - b -) phenotype, which differs from the African type.
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M3 - Article
C2 - 10885196
AN - SCOPUS:0034095819
SN - 0018-7143
VL - 72
SP - 511
EP - 518
JO - Human Biology
JF - Human Biology
IS - 3
ER -
