Serum-dependent potentiation of lipopolysaccharide-induced nitric oxide production is mediated by the events after the transcription of inducible type of nitric oxide synthase

Yue H. Zhang, William J. Murphy, Stephen W. Russell, David C. Morrison, Naoki Koide, Tomoaki Yoshida, Takashi Yokochi

Research output: Contribution to journalArticlepeer-review

4 Citations (Scopus)

Abstract

The mechanism of serum-dependent potentiation of lipopolysaccharide (LPS)-induced nitric oxide (NO) production was studied by incubating mouse macrophage cell line, RAW 264.7, in the presence of fetal bovine serum (FBS). The addition of FBS definitely enhanced LPS-induced NO production through augmented expression of inducible type NO synthase (iNOS) mRNA and protein. However, nuclear run-on analysis demonstrated only marginal enhancement in the rate of LPS-induced iNOS gene transcription in the presence of FBS. Further, there was no significant difference in the luciferase reporter gene activity linked to the iNOS promoter-enhancer gene in response to LPS between the presence and absence of FBS. FBS-dependent enhancement did not appear to involve the initial step for triggering iNOS transcription in LPS-induced NO production. Rather, FBS was suggested to affect the accumulation and stabilization of iNOS mRNA leading to iNOS protein and NO production by some post-transcriptional regulatory mechanism.

Original languageEnglish
Pages (from-to)16-22
Number of pages7
JournalCellular Immunology
Volume234
Issue number1
DOIs
Publication statusPublished - 03-2005

All Science Journal Classification (ASJC) codes

  • Immunology

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