Sex differences in metabolism of trichloroethylene and trichloroethanol in guinea pigs

Yui Hibino, Hailan Wang, Hisao Naito, Na Zhao, Dong Wang, Xiaofang Jia, Hongling Li, Xiangrong Song, Lili Liu, Yongshun Huang, Yuki Ito, Hanlin Huang, Michihiro Kamijima, Tamie Nakajima

Research output: Contribution to journalArticle

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Abstract

Sex Differences in Metabolism of Trichloroethylene and Trichloroethanol in Guinea Pigs: Yui HIBINO, et al. Department of Occupational and Environmental Health, Nagoya University Graduate School of Medicine-Objectives: Trichloroethylene (TRI) has the potential to cause generalized dermatitis complicated with hepatitis. The guinea pig maximization test (GPMT) also suggests that both TRI and its metabolite trichloroethanol (TCE) exhibit immunogenicity and possible sex differences in guinea pigs. However, TRI and TCE metabolisms in guinea pigs have not been elucidated in detail. The first issue to clarify may be the sex differences in relation to the immunogenicity. Methods: We collected urine from Hartley male and female guinea pigs 24 hours after intracutaneous injection of TRI, TCE or trichloroacetic acid (TCA) during a GPMT and measured the urinary metabolites by gas chromatography-mass spectrometry. Results: After TRI treatment, the amount of TCA was significantly greater in females than males, while there was no sex difference in the total amount (TCA + TCE). TCA was only detected in urine after TCA treatment. Interestingly, not only TCE but also TCA was detected in urine of both sexes after TCE treatment, and the amount of TCA was also greater in females than males. An additional experiment showed that TCE treatment did not result in the detection of urinary TCA in cytochrome P450 (CYP)2E1-null mice but did in wild-type mice, suggesting the involvement of CYP2E1 in the metabolism from TCE to TCA. The constitutive expression of CYP2E1 in the liver of guinea pigs was greater in females than males. Conclusions: The sex difference in urinary TCA excretion after TRI and TCE treatments may be due to variation of the constitutive expression of CYP2E1.

Original languageEnglish
Pages (from-to)443-449
Number of pages7
JournalJournal of Occupational Health
Volume55
Issue number6
DOIs
Publication statusPublished - 01-01-2013

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Trichloroethylene
Trichloroacetic Acid
Sex Characteristics
Guinea Pigs
Cytochrome P-450 CYP2E1
Urine
2,2,2-trichloroethanol
Therapeutics
Environmental Health
Dermatitis
Occupational Health
Gas Chromatography-Mass Spectrometry
Hepatitis
Medicine

All Science Journal Classification (ASJC) codes

  • Public Health, Environmental and Occupational Health

Cite this

Hibino, Yui ; Wang, Hailan ; Naito, Hisao ; Zhao, Na ; Wang, Dong ; Jia, Xiaofang ; Li, Hongling ; Song, Xiangrong ; Liu, Lili ; Huang, Yongshun ; Ito, Yuki ; Huang, Hanlin ; Kamijima, Michihiro ; Nakajima, Tamie. / Sex differences in metabolism of trichloroethylene and trichloroethanol in guinea pigs. In: Journal of Occupational Health. 2013 ; Vol. 55, No. 6. pp. 443-449.
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abstract = "Sex Differences in Metabolism of Trichloroethylene and Trichloroethanol in Guinea Pigs: Yui HIBINO, et al. Department of Occupational and Environmental Health, Nagoya University Graduate School of Medicine-Objectives: Trichloroethylene (TRI) has the potential to cause generalized dermatitis complicated with hepatitis. The guinea pig maximization test (GPMT) also suggests that both TRI and its metabolite trichloroethanol (TCE) exhibit immunogenicity and possible sex differences in guinea pigs. However, TRI and TCE metabolisms in guinea pigs have not been elucidated in detail. The first issue to clarify may be the sex differences in relation to the immunogenicity. Methods: We collected urine from Hartley male and female guinea pigs 24 hours after intracutaneous injection of TRI, TCE or trichloroacetic acid (TCA) during a GPMT and measured the urinary metabolites by gas chromatography-mass spectrometry. Results: After TRI treatment, the amount of TCA was significantly greater in females than males, while there was no sex difference in the total amount (TCA + TCE). TCA was only detected in urine after TCA treatment. Interestingly, not only TCE but also TCA was detected in urine of both sexes after TCE treatment, and the amount of TCA was also greater in females than males. An additional experiment showed that TCE treatment did not result in the detection of urinary TCA in cytochrome P450 (CYP)2E1-null mice but did in wild-type mice, suggesting the involvement of CYP2E1 in the metabolism from TCE to TCA. The constitutive expression of CYP2E1 in the liver of guinea pigs was greater in females than males. Conclusions: The sex difference in urinary TCA excretion after TRI and TCE treatments may be due to variation of the constitutive expression of CYP2E1.",
author = "Yui Hibino and Hailan Wang and Hisao Naito and Na Zhao and Dong Wang and Xiaofang Jia and Hongling Li and Xiangrong Song and Lili Liu and Yongshun Huang and Yuki Ito and Hanlin Huang and Michihiro Kamijima and Tamie Nakajima",
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Hibino, Y, Wang, H, Naito, H, Zhao, N, Wang, D, Jia, X, Li, H, Song, X, Liu, L, Huang, Y, Ito, Y, Huang, H, Kamijima, M & Nakajima, T 2013, 'Sex differences in metabolism of trichloroethylene and trichloroethanol in guinea pigs', Journal of Occupational Health, vol. 55, no. 6, pp. 443-449. https://doi.org/10.1539/joh.13-0091-OA

Sex differences in metabolism of trichloroethylene and trichloroethanol in guinea pigs. / Hibino, Yui; Wang, Hailan; Naito, Hisao; Zhao, Na; Wang, Dong; Jia, Xiaofang; Li, Hongling; Song, Xiangrong; Liu, Lili; Huang, Yongshun; Ito, Yuki; Huang, Hanlin; Kamijima, Michihiro; Nakajima, Tamie.

In: Journal of Occupational Health, Vol. 55, No. 6, 01.01.2013, p. 443-449.

Research output: Contribution to journalArticle

TY - JOUR

T1 - Sex differences in metabolism of trichloroethylene and trichloroethanol in guinea pigs

AU - Hibino, Yui

AU - Wang, Hailan

AU - Naito, Hisao

AU - Zhao, Na

AU - Wang, Dong

AU - Jia, Xiaofang

AU - Li, Hongling

AU - Song, Xiangrong

AU - Liu, Lili

AU - Huang, Yongshun

AU - Ito, Yuki

AU - Huang, Hanlin

AU - Kamijima, Michihiro

AU - Nakajima, Tamie

PY - 2013/1/1

Y1 - 2013/1/1

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