Abstract
Cutaneous immune reactions are known to show sexual dimorphism. Langerhans cells (LCs) are bone marrow-derived immune cells in the epidermis and are essential to immune reactions in the skin. In the present research, a study was made of the differences in LC density of male and female mice. Epidermal sheets were separated from the skin of the glabrous part of hind limbs and ears of specific pathogen-free (SPF) mice by ethylenediaminetetraacetic acid (EDTA) treatment and stained for adenosine triphosphatase (ATPase) activity. The density of LCs of hind limb epidermis in male C57BL/6 (823 ± 20/mm2) and BALB/c (1689 ± 66/mm2) mice was significantly less than that in females (1363 ± 52/mm2, p < 0.001; 2249 ± 105/mm2, p < 0.001, respectively). Langerhans cell density in the ears of male C57BL/6 (465 ± 24/mm2) mice was also significantly less than that in females (542 ± 17/mm2, p < 0.02). Although ovariectomy failed to bring about any change in the LC density of hind limb epidermis in female C57BL/6 mice, the LC density in male C57BL/6 mice increased significantly at 4 weeks following orchiectomy (sham operation, 564 ± 27/mm2; castration, 1179 ± 49/mm2, p < 0.001). These results indicate that mouse epidermal LC density depends on sex, i.e., male mice have fewer LCs than female mice. The reduction in LC density in males may possibly be caused by the testis.
| Original language | English |
|---|---|
| Pages (from-to) | 541-544 |
| Number of pages | 4 |
| Journal | Journal of Investigative Dermatology |
| Volume | 88 |
| Issue number | 5 |
| DOIs | |
| Publication status | Published - 01-01-1987 |
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All Science Journal Classification (ASJC) codes
- Biochemistry
- Molecular Biology
- Dermatology
- Cell Biology
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Sex differences in the densities of epidermal langerhans cells of the mouse. / Koyama, Yoh ichi; Nagao, Shizuko; Ohashi, Koji; Takahashi, Hisahide; Marunouchi, Tohru.
In: Journal of Investigative Dermatology, Vol. 88, No. 5, 01.01.1987, p. 541-544.Research output: Contribution to journal › Article
TY - JOUR
T1 - Sex differences in the densities of epidermal langerhans cells of the mouse
AU - Koyama, Yoh ichi
AU - Nagao, Shizuko
AU - Ohashi, Koji
AU - Takahashi, Hisahide
AU - Marunouchi, Tohru
PY - 1987/1/1
Y1 - 1987/1/1
N2 - Cutaneous immune reactions are known to show sexual dimorphism. Langerhans cells (LCs) are bone marrow-derived immune cells in the epidermis and are essential to immune reactions in the skin. In the present research, a study was made of the differences in LC density of male and female mice. Epidermal sheets were separated from the skin of the glabrous part of hind limbs and ears of specific pathogen-free (SPF) mice by ethylenediaminetetraacetic acid (EDTA) treatment and stained for adenosine triphosphatase (ATPase) activity. The density of LCs of hind limb epidermis in male C57BL/6 (823 ± 20/mm2) and BALB/c (1689 ± 66/mm2) mice was significantly less than that in females (1363 ± 52/mm2, p < 0.001; 2249 ± 105/mm2, p < 0.001, respectively). Langerhans cell density in the ears of male C57BL/6 (465 ± 24/mm2) mice was also significantly less than that in females (542 ± 17/mm2, p < 0.02). Although ovariectomy failed to bring about any change in the LC density of hind limb epidermis in female C57BL/6 mice, the LC density in male C57BL/6 mice increased significantly at 4 weeks following orchiectomy (sham operation, 564 ± 27/mm2; castration, 1179 ± 49/mm2, p < 0.001). These results indicate that mouse epidermal LC density depends on sex, i.e., male mice have fewer LCs than female mice. The reduction in LC density in males may possibly be caused by the testis.
AB - Cutaneous immune reactions are known to show sexual dimorphism. Langerhans cells (LCs) are bone marrow-derived immune cells in the epidermis and are essential to immune reactions in the skin. In the present research, a study was made of the differences in LC density of male and female mice. Epidermal sheets were separated from the skin of the glabrous part of hind limbs and ears of specific pathogen-free (SPF) mice by ethylenediaminetetraacetic acid (EDTA) treatment and stained for adenosine triphosphatase (ATPase) activity. The density of LCs of hind limb epidermis in male C57BL/6 (823 ± 20/mm2) and BALB/c (1689 ± 66/mm2) mice was significantly less than that in females (1363 ± 52/mm2, p < 0.001; 2249 ± 105/mm2, p < 0.001, respectively). Langerhans cell density in the ears of male C57BL/6 (465 ± 24/mm2) mice was also significantly less than that in females (542 ± 17/mm2, p < 0.02). Although ovariectomy failed to bring about any change in the LC density of hind limb epidermis in female C57BL/6 mice, the LC density in male C57BL/6 mice increased significantly at 4 weeks following orchiectomy (sham operation, 564 ± 27/mm2; castration, 1179 ± 49/mm2, p < 0.001). These results indicate that mouse epidermal LC density depends on sex, i.e., male mice have fewer LCs than female mice. The reduction in LC density in males may possibly be caused by the testis.
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U2 - 10.1111/1523-1747.ep12470104
DO - 10.1111/1523-1747.ep12470104
M3 - Article
C2 - 3572027
AN - SCOPUS:0023195249
VL - 88
SP - 541
EP - 544
JO - Journal of Investigative Dermatology
JF - Journal of Investigative Dermatology
SN - 0022-202X
IS - 5
ER -