Sex differences in the densities of epidermal langerhans cells of the mouse

Yoh ichi Koyama, Shizuko Nagao, Koji Ohashi, Hisahide Takahashi, Tohru Marunouchi

Research output: Contribution to journalArticle

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Abstract

Cutaneous immune reactions are known to show sexual dimorphism. Langerhans cells (LCs) are bone marrow-derived immune cells in the epidermis and are essential to immune reactions in the skin. In the present research, a study was made of the differences in LC density of male and female mice. Epidermal sheets were separated from the skin of the glabrous part of hind limbs and ears of specific pathogen-free (SPF) mice by ethylenediaminetetraacetic acid (EDTA) treatment and stained for adenosine triphosphatase (ATPase) activity. The density of LCs of hind limb epidermis in male C57BL/6 (823 ± 20/mm2) and BALB/c (1689 ± 66/mm2) mice was significantly less than that in females (1363 ± 52/mm2, p < 0.001; 2249 ± 105/mm2, p < 0.001, respectively). Langerhans cell density in the ears of male C57BL/6 (465 ± 24/mm2) mice was also significantly less than that in females (542 ± 17/mm2, p < 0.02). Although ovariectomy failed to bring about any change in the LC density of hind limb epidermis in female C57BL/6 mice, the LC density in male C57BL/6 mice increased significantly at 4 weeks following orchiectomy (sham operation, 564 ± 27/mm2; castration, 1179 ± 49/mm2, p < 0.001). These results indicate that mouse epidermal LC density depends on sex, i.e., male mice have fewer LCs than female mice. The reduction in LC density in males may possibly be caused by the testis.

Original languageEnglish
Pages (from-to)541-544
Number of pages4
JournalJournal of Investigative Dermatology
Volume88
Issue number5
DOIs
Publication statusPublished - 01-01-1987

Fingerprint

Antigen-antibody reactions
Langerhans Cells
Sex Characteristics
Skin
Cells
Pathogens
Cell Count
Edetic Acid
Adenosine Triphosphatases
Bone
Epidermis
Extremities
Inbred C57BL Mouse
Ear
Specific Pathogen-Free Organisms
Orchiectomy
Castration
Ovariectomy
Testis
Bone Marrow

All Science Journal Classification (ASJC) codes

  • Biochemistry
  • Molecular Biology
  • Dermatology
  • Cell Biology

Cite this

Koyama, Yoh ichi ; Nagao, Shizuko ; Ohashi, Koji ; Takahashi, Hisahide ; Marunouchi, Tohru. / Sex differences in the densities of epidermal langerhans cells of the mouse. In: Journal of Investigative Dermatology. 1987 ; Vol. 88, No. 5. pp. 541-544.
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Sex differences in the densities of epidermal langerhans cells of the mouse. / Koyama, Yoh ichi; Nagao, Shizuko; Ohashi, Koji; Takahashi, Hisahide; Marunouchi, Tohru.

In: Journal of Investigative Dermatology, Vol. 88, No. 5, 01.01.1987, p. 541-544.

Research output: Contribution to journalArticle

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