Sialic acid sulfation is induced by the antibiotic treatment in mammalian cells

Nursah Ertunc, Chihiro Sato, Ken Kitajima

Research output: Contribution to journalArticlepeer-review

3 Citations (Scopus)

Abstract

Sialic acids (Sias) are an outermost-situated sugar of glycoproteins and glycolipids to play important roles in various biological phenomena. They are often modified by additional substituents, such as O-acetyl group, to display more than 50 different structures in nature. Of those modified Sia, nothing is known about the occurrence and biological functions of sulfated Sias (SiaSs) in mammals. To elucidate the significance of sialic acid sulfation, we investigated various mammalian-cultured cell lines for the expression of SiaS using the specific antibody 3G9. First, SiaS is expressed in a cell line-dependent and a cell density-dependent manner. Second, in CHO cells, the expression of SiaS is reversibly induced by treatment with the antibiotic G418. Taken together, the expression of SiaS is changed by intrinsic and extrinsic factors in mammalian cells. This is the first demonstration of regulated expression of SiaS.

Original languageEnglish
Pages (from-to)2311-2318
Number of pages8
JournalBioscience, Biotechnology and Biochemistry
Volume84
Issue number11
DOIs
Publication statusPublished - 01-11-2020
Externally publishedYes

All Science Journal Classification (ASJC) codes

  • Biotechnology
  • Analytical Chemistry
  • Biochemistry
  • Applied Microbiology and Biotechnology
  • Molecular Biology
  • Organic Chemistry

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