TY - JOUR
T1 - Sialic acid sulfation is induced by the antibiotic treatment in mammalian cells
AU - Ertunc, Nursah
AU - Sato, Chihiro
AU - Kitajima, Ken
N1 - Funding Information:
This work was in part supported by a Grant-in-Aid for Scientific Research (C) [19K05812; to KK]; Kobayashi Foundation 7th Research Grant [to KK].
Publisher Copyright:
© 2020 Japan Society for Bioscience, Biotechnology, and Agrochemistry.
PY - 2020/11/1
Y1 - 2020/11/1
N2 - Sialic acids (Sias) are an outermost-situated sugar of glycoproteins and glycolipids to play important roles in various biological phenomena. They are often modified by additional substituents, such as O-acetyl group, to display more than 50 different structures in nature. Of those modified Sia, nothing is known about the occurrence and biological functions of sulfated Sias (SiaSs) in mammals. To elucidate the significance of sialic acid sulfation, we investigated various mammalian-cultured cell lines for the expression of SiaS using the specific antibody 3G9. First, SiaS is expressed in a cell line-dependent and a cell density-dependent manner. Second, in CHO cells, the expression of SiaS is reversibly induced by treatment with the antibiotic G418. Taken together, the expression of SiaS is changed by intrinsic and extrinsic factors in mammalian cells. This is the first demonstration of regulated expression of SiaS.
AB - Sialic acids (Sias) are an outermost-situated sugar of glycoproteins and glycolipids to play important roles in various biological phenomena. They are often modified by additional substituents, such as O-acetyl group, to display more than 50 different structures in nature. Of those modified Sia, nothing is known about the occurrence and biological functions of sulfated Sias (SiaSs) in mammals. To elucidate the significance of sialic acid sulfation, we investigated various mammalian-cultured cell lines for the expression of SiaS using the specific antibody 3G9. First, SiaS is expressed in a cell line-dependent and a cell density-dependent manner. Second, in CHO cells, the expression of SiaS is reversibly induced by treatment with the antibiotic G418. Taken together, the expression of SiaS is changed by intrinsic and extrinsic factors in mammalian cells. This is the first demonstration of regulated expression of SiaS.
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U2 - 10.1080/09168451.2020.1792763
DO - 10.1080/09168451.2020.1792763
M3 - Article
C2 - 32752946
AN - SCOPUS:85089034445
VL - 84
SP - 2311
EP - 2318
JO - Bioscience, Biotechnology and Biochemistry
JF - Bioscience, Biotechnology and Biochemistry
SN - 0916-8451
IS - 11
ER -