TY - JOUR
T1 - Sigma region located between C, and C, genes of human immunoglobulin heavy chain
T2 - Possible involvement of tRNA-like structure in RNA splicing
AU - Akahori, Yasushi
AU - Handa, Hiroshi
AU - Imai, Kenji
AU - Abe, Masumi
AU - Kameyama, Kohzoh
AU - Hibiya, Makoto
AU - Yasui, Hisashi
AU - Okamura, Kazuhiko
AU - Naito, Morihiro
AU - Matsuoka, Hiroshi
AU - Kurosawa, Yoshikazu
N1 - Funding Information:
ACKNOWLEDGEMENTS We are grateful to Dr.S.Ohno for critical reading of the manuscript. We thank Drs.L.Hood, P.Leder, S.Tonegawa, T.Maniatis and M.Muramatsu for providing us clones 5A, 5D, CH4-38, CH4-51, MEP12, human DNA library and RNA polymerase I promoter, respectively. We also are grateful to Drs.T.Okazaki, Y.Takagi, I.Ishiguro and K.Fujita for their encouragement and to Ms. A.Nagata for preparation of the manuscript. This work was supported in part by grants from the Ministry of Education, Science and Culture of Japan, and Fujita-Gakuen Health University, Japan Private School Promotion Foundation and the Uehara Science Foundation.
PY - 1988/10/25
Y1 - 1988/10/25
N2 - Noncoding regions within the cluster of immunoglobulin heavy chain constant genes in the human genome contained a number of repeats. In the μ-δ intron, two repeating units were contained. One 442-base-long fragment located JH-μ intron ( defined as "sigma μ(σμ)") occupied the position in the μ-σ intron. The other 1166-base-long fragment located somewhere in front of S (class switch) region of Cγ gene was also found in the μ-δ intron. We defined the repeats in the μ-δ intron as "SIGMA (Σ)". The polarities of the longer repeats in the genome were opposite between the μ-δ intron and the upstreams of Cγ genes. These inverted copies (defined as σγ3 and σγ4), located 6 kb upstream of their respective Cγ's, were apparently transcribed in vitro, via RNA polymerase III and transcripts should have contained tRNA-like structures. Small DNA fragments capable of encoding tRNA-like structures were also found in corresponding regions of mouse Ig Cγ cluster.
AB - Noncoding regions within the cluster of immunoglobulin heavy chain constant genes in the human genome contained a number of repeats. In the μ-δ intron, two repeating units were contained. One 442-base-long fragment located JH-μ intron ( defined as "sigma μ(σμ)") occupied the position in the μ-σ intron. The other 1166-base-long fragment located somewhere in front of S (class switch) region of Cγ gene was also found in the μ-δ intron. We defined the repeats in the μ-δ intron as "SIGMA (Σ)". The polarities of the longer repeats in the genome were opposite between the μ-δ intron and the upstreams of Cγ genes. These inverted copies (defined as σγ3 and σγ4), located 6 kb upstream of their respective Cγ's, were apparently transcribed in vitro, via RNA polymerase III and transcripts should have contained tRNA-like structures. Small DNA fragments capable of encoding tRNA-like structures were also found in corresponding regions of mouse Ig Cγ cluster.
UR - https://www.scopus.com/pages/publications/0023716702
UR - https://www.scopus.com/pages/publications/0023716702#tab=citedBy
U2 - 10.1093/nar/16.20.9497
DO - 10.1093/nar/16.20.9497
M3 - Article
C2 - 3141902
AN - SCOPUS:0023716702
SN - 0305-1048
VL - 16
SP - 9497
EP - 9511
JO - Nucleic acids research
JF - Nucleic acids research
IS - 20
ER -