Significance of downregulation of renal organic cation transporter (SLC47A1) in cisplatin-induced proximal tubular injury

Tomohiro Mizuno, Waichi Sato, Kazuhiro Ishikawa, Yuki Terao, Kazuo Takahashi, Yukihiro Noda, Yukio Yuzawa, Tadashi Nagamatsu

Research output: Contribution to journalArticle

Abstract

Background/aim: To elucidate the mechanism responsible for developing acute kidney injury in patients with diabetes mellitus, we also evaluated the issue of whether advanced glycation endproducts (AGEs) influence the expressions of multi antimicrobial extrusion protein (MATE1/SLC47A1) in tubular cells. Materials and methods: To detect changing expression of MATE1/SLC47A1 in dose- and time-dependent manners, human proximal tubular epithelial cells were incubated with AGE-aggregated-human serum albumin. As a function assay for MATE1/SLC47A1, human proximal tubular epithelial cells were incubated with cisplatin or carboplatin. Results: On incubation with AGEs, the expressions of MATE1/SLC47A1 were decreased in tubular cells. In addition, the toxicities of cisplatin were increased in tubular cells that had been pretreated with AGEs. However, the toxicities of carboplatin were smaller than that of cisplatin in proximal tubular epithelial cells. Conclusion: The expression of the MATE1/SLC47A1 is decreased by AGEs, which increases the risk for proximal tubular injury.

Original languageEnglish
Article numberA198
Pages (from-to)1701-1706
Number of pages6
JournalOncoTargets and Therapy
Volume8
DOIs
Publication statusPublished - 10-07-2015

Fingerprint

Cisplatin
Cations
Down-Regulation
Epithelial Cells
Carboplatin
Kidney
Wounds and Injuries
Acute Kidney Injury
Serum Albumin
Diabetes Mellitus
Proteins

All Science Journal Classification (ASJC) codes

  • Oncology
  • Pharmacology (medical)

Cite this

Mizuno, Tomohiro ; Sato, Waichi ; Ishikawa, Kazuhiro ; Terao, Yuki ; Takahashi, Kazuo ; Noda, Yukihiro ; Yuzawa, Yukio ; Nagamatsu, Tadashi. / Significance of downregulation of renal organic cation transporter (SLC47A1) in cisplatin-induced proximal tubular injury. In: OncoTargets and Therapy. 2015 ; Vol. 8. pp. 1701-1706.
@article{f07bcc3f03774fffaa96a3eb8d8bbf38,
title = "Significance of downregulation of renal organic cation transporter (SLC47A1) in cisplatin-induced proximal tubular injury",
abstract = "Background/aim: To elucidate the mechanism responsible for developing acute kidney injury in patients with diabetes mellitus, we also evaluated the issue of whether advanced glycation endproducts (AGEs) influence the expressions of multi antimicrobial extrusion protein (MATE1/SLC47A1) in tubular cells. Materials and methods: To detect changing expression of MATE1/SLC47A1 in dose- and time-dependent manners, human proximal tubular epithelial cells were incubated with AGE-aggregated-human serum albumin. As a function assay for MATE1/SLC47A1, human proximal tubular epithelial cells were incubated with cisplatin or carboplatin. Results: On incubation with AGEs, the expressions of MATE1/SLC47A1 were decreased in tubular cells. In addition, the toxicities of cisplatin were increased in tubular cells that had been pretreated with AGEs. However, the toxicities of carboplatin were smaller than that of cisplatin in proximal tubular epithelial cells. Conclusion: The expression of the MATE1/SLC47A1 is decreased by AGEs, which increases the risk for proximal tubular injury.",
author = "Tomohiro Mizuno and Waichi Sato and Kazuhiro Ishikawa and Yuki Terao and Kazuo Takahashi and Yukihiro Noda and Yukio Yuzawa and Tadashi Nagamatsu",
year = "2015",
month = "7",
day = "10",
doi = "10.2147/OTT.S86743",
language = "English",
volume = "8",
pages = "1701--1706",
journal = "OncoTargets and Therapy",
issn = "1178-6930",
publisher = "Dove Medical Press Ltd.",

}

Significance of downregulation of renal organic cation transporter (SLC47A1) in cisplatin-induced proximal tubular injury. / Mizuno, Tomohiro; Sato, Waichi; Ishikawa, Kazuhiro; Terao, Yuki; Takahashi, Kazuo; Noda, Yukihiro; Yuzawa, Yukio; Nagamatsu, Tadashi.

In: OncoTargets and Therapy, Vol. 8, A198, 10.07.2015, p. 1701-1706.

Research output: Contribution to journalArticle

TY - JOUR

T1 - Significance of downregulation of renal organic cation transporter (SLC47A1) in cisplatin-induced proximal tubular injury

AU - Mizuno, Tomohiro

AU - Sato, Waichi

AU - Ishikawa, Kazuhiro

AU - Terao, Yuki

AU - Takahashi, Kazuo

AU - Noda, Yukihiro

AU - Yuzawa, Yukio

AU - Nagamatsu, Tadashi

PY - 2015/7/10

Y1 - 2015/7/10

N2 - Background/aim: To elucidate the mechanism responsible for developing acute kidney injury in patients with diabetes mellitus, we also evaluated the issue of whether advanced glycation endproducts (AGEs) influence the expressions of multi antimicrobial extrusion protein (MATE1/SLC47A1) in tubular cells. Materials and methods: To detect changing expression of MATE1/SLC47A1 in dose- and time-dependent manners, human proximal tubular epithelial cells were incubated with AGE-aggregated-human serum albumin. As a function assay for MATE1/SLC47A1, human proximal tubular epithelial cells were incubated with cisplatin or carboplatin. Results: On incubation with AGEs, the expressions of MATE1/SLC47A1 were decreased in tubular cells. In addition, the toxicities of cisplatin were increased in tubular cells that had been pretreated with AGEs. However, the toxicities of carboplatin were smaller than that of cisplatin in proximal tubular epithelial cells. Conclusion: The expression of the MATE1/SLC47A1 is decreased by AGEs, which increases the risk for proximal tubular injury.

AB - Background/aim: To elucidate the mechanism responsible for developing acute kidney injury in patients with diabetes mellitus, we also evaluated the issue of whether advanced glycation endproducts (AGEs) influence the expressions of multi antimicrobial extrusion protein (MATE1/SLC47A1) in tubular cells. Materials and methods: To detect changing expression of MATE1/SLC47A1 in dose- and time-dependent manners, human proximal tubular epithelial cells were incubated with AGE-aggregated-human serum albumin. As a function assay for MATE1/SLC47A1, human proximal tubular epithelial cells were incubated with cisplatin or carboplatin. Results: On incubation with AGEs, the expressions of MATE1/SLC47A1 were decreased in tubular cells. In addition, the toxicities of cisplatin were increased in tubular cells that had been pretreated with AGEs. However, the toxicities of carboplatin were smaller than that of cisplatin in proximal tubular epithelial cells. Conclusion: The expression of the MATE1/SLC47A1 is decreased by AGEs, which increases the risk for proximal tubular injury.

UR - http://www.scopus.com/inward/record.url?scp=84937696912&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84937696912&partnerID=8YFLogxK

U2 - 10.2147/OTT.S86743

DO - 10.2147/OTT.S86743

M3 - Article

VL - 8

SP - 1701

EP - 1706

JO - OncoTargets and Therapy

JF - OncoTargets and Therapy

SN - 1178-6930

M1 - A198

ER -