TY - JOUR
T1 - Silencing of FUS in the common marmoset (Callithrix jacchus) brain via stereotaxic injection of an adeno-associated virus encoding shRNA
AU - Endo, Kuniyuki
AU - Ishigaki, Shinsuke
AU - Masamizu, Yoshito
AU - Fujioka, Yusuke
AU - Watakabe, Akiya
AU - Yamamori, Tetsuo
AU - Hatanaka, Nobuhiko
AU - Nambu, Atsushi
AU - Okado, Haruo
AU - Katsuno, Masahisa
AU - Watanabe, Hirohisa
AU - Matsuzaki, Masanori
AU - Sobue, Gen
N1 - Publisher Copyright:
© 2017 Elsevier Ireland Ltd and Japan Neuroscience Society
PY - 2018/5
Y1 - 2018/5
N2 - Fused in sarcoma (FUS) is an RNA binding protein that is involved in frontotemporal lobar degeneration (FTLD) and amyotrophic lateral sclerosis (ALS). To establish the common marmoset (Callithrix jacchus) as a model for FTLD, we generated a stereotaxic injection-based marmoset model of FUS-silencing. We designed shRNAs against the marmoset FUS gene and generated an AAV9 virus encoding the most effective shRNA against FUS (shFUS). The AAV encoding shFUS (AAV-shFUS) was introduced into the frontal cortex of young adult marmosets, whereas AAV encoding a control shRNA was injected into the contralateral side. We obtained approximately 70–80% silencing of FUS following AAV-shFUS injection. Interestingly, FUS-silencing provoked a proliferation of astrocytes and microglias. Since FTLD is characterized by various emotional deficits, it would be helpful to establish a marmoset model of FUS-silencing in various brain tissues for investigating the pathomechanism of higher cognitive and behavioral dysfunction.
AB - Fused in sarcoma (FUS) is an RNA binding protein that is involved in frontotemporal lobar degeneration (FTLD) and amyotrophic lateral sclerosis (ALS). To establish the common marmoset (Callithrix jacchus) as a model for FTLD, we generated a stereotaxic injection-based marmoset model of FUS-silencing. We designed shRNAs against the marmoset FUS gene and generated an AAV9 virus encoding the most effective shRNA against FUS (shFUS). The AAV encoding shFUS (AAV-shFUS) was introduced into the frontal cortex of young adult marmosets, whereas AAV encoding a control shRNA was injected into the contralateral side. We obtained approximately 70–80% silencing of FUS following AAV-shFUS injection. Interestingly, FUS-silencing provoked a proliferation of astrocytes and microglias. Since FTLD is characterized by various emotional deficits, it would be helpful to establish a marmoset model of FUS-silencing in various brain tissues for investigating the pathomechanism of higher cognitive and behavioral dysfunction.
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U2 - 10.1016/j.neures.2017.08.006
DO - 10.1016/j.neures.2017.08.006
M3 - Article
C2 - 28842245
AN - SCOPUS:85028604320
SN - 0168-0102
VL - 130
SP - 56
EP - 64
JO - Neuroscience Research
JF - Neuroscience Research
ER -