Silibinin prevents amyloid b peptide-induced memory impairment and oxidative stress in mice

P. Lu, T. Mamiya, L. L. Lu, Akihiro Mouri, L. B. Zou, T. Nagai, M. Hiramatsu, T. Ikejima, Toshitaka Nabeshima

Research output: Contribution to journalArticle

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Abstract

Background and purpose: Accumulated evidence suggests that oxidative stress is involved in amyloid b (Ab)-induced cognitive dysfunction. Silibinin (silybin), a flavonoid derived from the herb milk thistle (Silybum marianum), has been shown to have antioxidative properties; however, it remains unclear whether silibinin improves Ab-induced neurotoxicity. In the present study, we examined the effect of silibinin on the memory impairment and accumulation of oxidative stress induced by Ab25-35 in mice. Experimental approach: Aggregated Ab25-35 (3 nmol) was intracerebroventricularly administered to mice. Treatment with silibinin (2, 20 and 200 mg-kg-1, once a day, p.o.) was started immediately after the injection of Ab25-35. Locomotor activity was evaluated 6 days after the Ab25-35 treatment, and cognitive function was evaluated in a Y-maze and novel object recognition tests 6-11 days after the Ab25-35 treatment. The levels of lipid peroxidation (malondialdehyde) and antioxidant (glutathione) in the hippocampus were measured 7 days after the Ab25-35 injection. Key results: Silibinin prevented the memory impairment induced by Ab25-35 in the Y-maze and novel object recognition tests. Repeated treatment with silibinin attenuated the Ab25-35-induced accumulation of malondialdehyde and depletion of glutathione in the hippocampus. Conclusions and implications: Silibinin prevents memory impairment and oxidative damage induced by Ab25-35 and may be a potential therapeutic agent for Alzheimer's disease.

Original languageEnglish
Pages (from-to)1270-1277
Number of pages8
JournalBritish Journal of Pharmacology
Volume157
Issue number7
DOIs
Publication statusPublished - 29-10-2009

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Amyloid
Oxidative Stress
Peptides
Milk Thistle
Malondialdehyde
Glutathione
Hippocampus
Therapeutics
Injections
silybin
Locomotion
Flavonoids
Cognition
Lipid Peroxidation
Alzheimer Disease
Antioxidants

All Science Journal Classification (ASJC) codes

  • Pharmacology

Cite this

Lu, P. ; Mamiya, T. ; Lu, L. L. ; Mouri, Akihiro ; Zou, L. B. ; Nagai, T. ; Hiramatsu, M. ; Ikejima, T. ; Nabeshima, Toshitaka. / Silibinin prevents amyloid b peptide-induced memory impairment and oxidative stress in mice. In: British Journal of Pharmacology. 2009 ; Vol. 157, No. 7. pp. 1270-1277.
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Silibinin prevents amyloid b peptide-induced memory impairment and oxidative stress in mice. / Lu, P.; Mamiya, T.; Lu, L. L.; Mouri, Akihiro; Zou, L. B.; Nagai, T.; Hiramatsu, M.; Ikejima, T.; Nabeshima, Toshitaka.

In: British Journal of Pharmacology, Vol. 157, No. 7, 29.10.2009, p. 1270-1277.

Research output: Contribution to journalArticle

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AU - Lu, P.

AU - Mamiya, T.

AU - Lu, L. L.

AU - Mouri, Akihiro

AU - Zou, L. B.

AU - Nagai, T.

AU - Hiramatsu, M.

AU - Ikejima, T.

AU - Nabeshima, Toshitaka

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