Simple disk-based method for detection of Klebsiella pneumoniae carbapenemase-type β-lactamase by use of a boronic acid compound

Yohei Doi, Brian A. Potoski, Jennifer M. Adams-Haduch, Hanna E. Sidjabat, Anthony W. Pasculle, David L. Paterson

Research output: Contribution to journalArticle

92 Citations (Scopus)

Abstract

A disk potentiation method using carbapenems as substrates and 3-aminophenyl boronic acid as an inhibitor was evaluated for the detection of Klebsiella pneumoniae carbapenemase (KPC)-type β-lactamases. When combined with nonsusceptibility to ertapenem, the method was easy to perform and reliably differentiated isolates producing KPC-type β-lactamases from those producing other types of β-lactamases.

Original languageEnglish
Pages (from-to)4083-4086
Number of pages4
JournalJournal of clinical microbiology
Volume46
Issue number12
DOIs
Publication statusPublished - 01-12-2008
Externally publishedYes

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Boronic Acids
Klebsiella pneumoniae
Carbapenems
carbapenemase
ertapenem

All Science Journal Classification (ASJC) codes

  • Microbiology (medical)

Cite this

Doi, Yohei ; Potoski, Brian A. ; Adams-Haduch, Jennifer M. ; Sidjabat, Hanna E. ; Pasculle, Anthony W. ; Paterson, David L. / Simple disk-based method for detection of Klebsiella pneumoniae carbapenemase-type β-lactamase by use of a boronic acid compound. In: Journal of clinical microbiology. 2008 ; Vol. 46, No. 12. pp. 4083-4086.
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Simple disk-based method for detection of Klebsiella pneumoniae carbapenemase-type β-lactamase by use of a boronic acid compound. / Doi, Yohei; Potoski, Brian A.; Adams-Haduch, Jennifer M.; Sidjabat, Hanna E.; Pasculle, Anthony W.; Paterson, David L.

In: Journal of clinical microbiology, Vol. 46, No. 12, 01.12.2008, p. 4083-4086.

Research output: Contribution to journalArticle

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