Simple disk-based method for detection of Klebsiella pneumoniae carbapenemase-type β-lactamase by use of a boronic acid compound

Yohei Doi; Brian A. Potoski; Jennifer M. Adams-Haduch; Hanna E. Sidjabat; Anthony W. Pasculle; David L. Paterson

doi:10.1128/JCM.01408-08

Simple disk-based method for detection of Klebsiella pneumoniae carbapenemase-type β-lactamase by use of a boronic acid compound

Yohei Doi
, Brian A. Potoski
, Jennifer M. Adams-Haduch
, Hanna E. Sidjabat
, Anthony W. Pasculle
, David L. Paterson

Research output: Contribution to journal › Article › peer-review

143 Citations (Scopus)

Abstract

A disk potentiation method using carbapenems as substrates and 3-aminophenyl boronic acid as an inhibitor was evaluated for the detection of Klebsiella pneumoniae carbapenemase (KPC)-type β-lactamases. When combined with nonsusceptibility to ertapenem, the method was easy to perform and reliably differentiated isolates producing KPC-type β-lactamases from those producing other types of β-lactamases.

Original language	English
Pages (from-to)	4083-4086
Number of pages	4
Journal	Journal of clinical microbiology
Volume	46
Issue number	12
DOIs	https://doi.org/10.1128/JCM.01408-08
Publication status	Published - 12-2008
Externally published	Yes

All Science Journal Classification (ASJC) codes

Microbiology (medical)

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10.1128/JCM.01408-08

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abstract = "A disk potentiation method using carbapenems as substrates and 3-aminophenyl boronic acid as an inhibitor was evaluated for the detection of Klebsiella pneumoniae carbapenemase (KPC)-type β-lactamases. When combined with nonsusceptibility to ertapenem, the method was easy to perform and reliably differentiated isolates producing KPC-type β-lactamases from those producing other types of β-lactamases.",

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AU - Doi, Yohei

AU - Potoski, Brian A.

AU - Adams-Haduch, Jennifer M.

AU - Sidjabat, Hanna E.

AU - Pasculle, Anthony W.

AU - Paterson, David L.

PY - 2008/12

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AB - A disk potentiation method using carbapenems as substrates and 3-aminophenyl boronic acid as an inhibitor was evaluated for the detection of Klebsiella pneumoniae carbapenemase (KPC)-type β-lactamases. When combined with nonsusceptibility to ertapenem, the method was easy to perform and reliably differentiated isolates producing KPC-type β-lactamases from those producing other types of β-lactamases.

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ER -

Simple disk-based method for detection of Klebsiella pneumoniae carbapenemase-type β-lactamase by use of a boronic acid compound

Abstract

All Science Journal Classification (ASJC) codes

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