Simple sandwich enzyme immunoassay for quantification of mouse mammary tumor virus in mouse milk

Masahide Takahashi; Shinsuke Saga; Shoichi Nagayoshi; Masao Imai; Yoshihiro Tsutsui; Munemitsu Hoshino

doi:10.1016/0304-3835(82)90056-8

Simple sandwich enzyme immunoassay for quantification of mouse mammary tumor virus in mouse milk

Masahide Takahashi
, Shinsuke Saga
, Shoichi Nagayoshi
, Masao Imai
, Yoshihiro Tsutsui
, Munemitsu Hoshino

Research output: Contribution to journal › Article › peer-review

1 Citation (Scopus)

Abstract

We have developed a sandwich enzyme immunoassay in order to measure quantitatively mouse mammary tumor virus (MMTV) in mouse milk. In this assay, the antibody-β-d-galactosidase complex and antibody-bound silicon rubber pieces as solid phase are used. The assay is able to detect 10 ng/ml of MMTV in the milk sample.

Original language	English
Pages (from-to)	155-161
Number of pages	7
Journal	Cancer Letters
Volume	16
Issue number	2
DOIs	https://doi.org/10.1016/0304-3835(82)90056-8
Publication status	Published - 1982
Externally published	Yes

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

SDG 3 Good Health and Well-being

All Science Journal Classification (ASJC) codes

Oncology
Cancer Research

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10.1016/0304-3835(82)90056-8

Cite this

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title = "Simple sandwich enzyme immunoassay for quantification of mouse mammary tumor virus in mouse milk",

abstract = "We have developed a sandwich enzyme immunoassay in order to measure quantitatively mouse mammary tumor virus (MMTV) in mouse milk. In this assay, the antibody-β-d-galactosidase complex and antibody-bound silicon rubber pieces as solid phase are used. The assay is able to detect 10 ng/ml of MMTV in the milk sample.",

author = "Masahide Takahashi and Shinsuke Saga and Shoichi Nagayoshi and Masao Imai and Yoshihiro Tsutsui and Munemitsu Hoshino",

note = "Funding Information: This work was supported in part by a grant-in-aid for cancer research from the Ministry of Education, Culture, and Science in Japan (No. 56010043 and No. 56010089).",

year = "1982",

doi = "10.1016/0304-3835(82)90056-8",

language = "English",

volume = "16",

pages = "155--161",

journal = "Cancer Letters",

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publisher = "Elsevier Ireland Ltd",

number = "2",

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TY - JOUR

T1 - Simple sandwich enzyme immunoassay for quantification of mouse mammary tumor virus in mouse milk

AU - Takahashi, Masahide

AU - Saga, Shinsuke

AU - Nagayoshi, Shoichi

AU - Imai, Masao

AU - Tsutsui, Yoshihiro

AU - Hoshino, Munemitsu

N1 - Funding Information: This work was supported in part by a grant-in-aid for cancer research from the Ministry of Education, Culture, and Science in Japan (No. 56010043 and No. 56010089).

PY - 1982

Y1 - 1982

N2 - We have developed a sandwich enzyme immunoassay in order to measure quantitatively mouse mammary tumor virus (MMTV) in mouse milk. In this assay, the antibody-β-d-galactosidase complex and antibody-bound silicon rubber pieces as solid phase are used. The assay is able to detect 10 ng/ml of MMTV in the milk sample.

AB - We have developed a sandwich enzyme immunoassay in order to measure quantitatively mouse mammary tumor virus (MMTV) in mouse milk. In this assay, the antibody-β-d-galactosidase complex and antibody-bound silicon rubber pieces as solid phase are used. The assay is able to detect 10 ng/ml of MMTV in the milk sample.

UR - https://www.scopus.com/pages/publications/0020322132

UR - https://www.scopus.com/pages/publications/0020322132#tab=citedBy

U2 - 10.1016/0304-3835(82)90056-8

DO - 10.1016/0304-3835(82)90056-8

M3 - Article

C2 - 6290029

AN - SCOPUS:0020322132

SN - 0304-3835

VL - 16

SP - 155

EP - 161

JO - Cancer Letters

JF - Cancer Letters

IS - 2

ER -

Simple sandwich enzyme immunoassay for quantification of mouse mammary tumor virus in mouse milk

Abstract

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