TY - JOUR
T1 - Simultaneous determinations of cefsulodin and cefotiam in serum and bone marrow blood by high‐performance liquid chromatography
AU - Yamamura, Keiko
AU - Nakao, Makoto
AU - Yamada, Jun‐Ichiro ‐I
AU - Yotsuyanagi, Toshihisa
PY - 1983/8
Y1 - 1983/8
N2 - A high‐performance liquid chromatographic method is described for the simultaneous determinations of cefsulodin and cefotiam in serum and bone marrow blood samples. After extraction with acetonitrile, the cephalosporins were applied to a reverse‐phase column with an internal standard, cefazolin; the mobile phase was a mixture of 0.005 M tetrabutylammonium phosphate and methanol (35:65, v/v). The method yielded satisfactory resolutions for these agents, and the results were compared with those obtained using the microbiological method. The statistical analysis of the relationship between the methods gave a good correlation for all of these agents and samples. The concentrations of cefsulodin and cefotiam, concurrently administered by the intravenous route to patients subjected to artificial total joint prosthesis, in serum and bone marrow blood collected at 0.5 and 1 hr postinjection were almost equivalent.
AB - A high‐performance liquid chromatographic method is described for the simultaneous determinations of cefsulodin and cefotiam in serum and bone marrow blood samples. After extraction with acetonitrile, the cephalosporins were applied to a reverse‐phase column with an internal standard, cefazolin; the mobile phase was a mixture of 0.005 M tetrabutylammonium phosphate and methanol (35:65, v/v). The method yielded satisfactory resolutions for these agents, and the results were compared with those obtained using the microbiological method. The statistical analysis of the relationship between the methods gave a good correlation for all of these agents and samples. The concentrations of cefsulodin and cefotiam, concurrently administered by the intravenous route to patients subjected to artificial total joint prosthesis, in serum and bone marrow blood collected at 0.5 and 1 hr postinjection were almost equivalent.
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U2 - 10.1002/jps.2600720833
DO - 10.1002/jps.2600720833
M3 - Article
C2 - 6312012
AN - SCOPUS:0020515683
SN - 0022-3549
VL - 72
SP - 958
EP - 960
JO - Journal of Pharmaceutical Sciences
JF - Journal of Pharmaceutical Sciences
IS - 8
ER -