Single chain variable fragment antibodies against shiga toxins isolated from a human antibody phage display library

Paola Neri, Naoko Shigemori, Susumu Hamada-Tsutsumi, Kentaro Tsukamoto, Hideyuki Arimitsu, Toshiyasu Shimizu, Yasushi Akahori, Yoshikazu Kurosawa, Takao Tsuji

Research output: Contribution to journalArticlepeer-review

14 Citations (Scopus)

Abstract

Shiga toxins (Stxs) are involved in the pathogenesis of hemolytic-uremic syndrome and other severe systemic complications following enterohemorrhagic Escherichia coli infection in humans. Passive immunotherapies using monoclonal antibodies have been shown to be effective for neutralizing the toxic effects of Stxs. However, animal-derived monoclonal antibodies are sometimes immunogenic and their production is both laborious and expensive. We here report the isolation of single-chain variable fragment antibodies against Stxs by screening a phage display library constructed from a naïve human repertoire. An antibody among the selected clones designated B22 bound to the binding subunits of both Stx-1 and Stx-2, and strongly neutralized the cytotoxicity of Stx-1. This is the first example of a monovalent antibody showing Stx-neutralizing activity. The B22 antibody is also completely naturally occurring in human, which reduces the possibility of adverse immunological effects, and can be easily produced using bacterial protein synthesis systems.

Original languageEnglish
Pages (from-to)5340-5346
Number of pages7
JournalVaccine
Volume29
Issue number33
DOIs
Publication statusPublished - 26-07-2011

All Science Journal Classification (ASJC) codes

  • Molecular Medicine
  • Immunology and Microbiology(all)
  • veterinary(all)
  • Public Health, Environmental and Occupational Health
  • Infectious Diseases

Fingerprint

Dive into the research topics of 'Single chain variable fragment antibodies against shiga toxins isolated from a human antibody phage display library'. Together they form a unique fingerprint.

Cite this