Single-tube multiplex polymerase chain reaction for the detection of genes encoding enterobacteriaceae carbapenemase

Masanori Watahiki, Ryuji Kawahara, Masahiro Suzuki, Miyako Aoki, Kaoru Uchida, Yuko Matsumoto, Yuko Kumagai, Makiko Noda, Kanako Masuda, Chiemi Fukuda, Seiya Harada, Keiko Senba, Masato Suzuki, Mari Matsui, Satowa Suzuki, Keigo Shibayama, Hiroto Shinomiya

Research output: Contribution to journalArticlepeer-review

2 Citations (Scopus)

Abstract

A multiplex PCR assay in a single tube was developed for the detection of the carbapenemase genes of Enterobacteriaceae. Primers were designed to amplify the following six carbapenemase genes: bla bla bla bla bla and bla Of 70 blaIMP variants, 67 subtypes were simulated toKPC,be PCR-positiveIMP,NDM,basedVIM,on in silicoOXA-48-like,simulation andGES.the primer-design strategy. After determining the optimal PCR conditions and performing in vitro assays, the performance of the PCR assay was evaluated using 51 and 91 clinical isolates with and without carbapenemase genes, respectively. In conclusion, the combination of multiplex PCR primers and QIAGEN Multiplex PCR Plus Kit was used to determine the best performance for the rapid and efficient screening of carbapenemase genes in Enterobacteriaceae. The assay had an overall sensitivity and specificity of 100%. This PCR assay compensates for the limitations of phenotypic testing, such as antimicrobial susceptibility testing and the modified carbapenem inactivation method, in clinical and public health settings.

Original languageEnglish
Pages (from-to)166-172
Number of pages7
JournalJapanese journal of infectious diseases
Volume73
Issue number2
DOIs
Publication statusPublished - 2020

All Science Journal Classification (ASJC) codes

  • Microbiology (medical)
  • Infectious Diseases

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