Small-Conductance Ca2+-Dependent K+ Channels Are the Target of Spike-Induced Ca2+ Release in a Feedback Regulation of Pyramidal Cell Excitability

Shin Ichiro Yamada, Hajime Takechi, Izumi Kanchiku, Toru Kita, Nobuo Kato

Research output: Contribution to journalArticle

33 Citations (Scopus)

Abstract

Cooperative regulation of inosiol-1,4,5-trisphosphate receptors (IP 3Rs) by Ca2+ and IP3 has been increasingly recognized, although its functional significance is not clear. The present experiments first confirmed that depolarization-induced Ca2+ influx triggers an outward current in visual cortex pyramidal cells in normal medium, which was mediated by apamin-sensitive, small-conductance Ca 2+-dependent K+ channels (SK channels). With IP 3-mobilizing neurotransmitters bath-applied, a delayed outward current was evoked in addition to the initial outward current and was mediated again by SK channels. Calcium turnover underlying this biphasic SK channel activation was investigated. By voltage-clamp recording, Ca2+ influx through voltage-dependent Ca2+ channels (VDCCs) was shown to be responsible for activating the initial SK current, whereas the IP3R blocker heparin abolished the delayed component. High-speed Ca2+ imaging revealed that a biphasic Ca2+ elevation indeed underlays this dual activation of SK channels. The first Ca2+ elevation originated from VDCCs, whereas the delayed phase was attributed to calcium release from IP3Rs. Such enhanced SK currents, activated dually by incoming and released calcium, were shown to intensify spike-frequency adaptation. We propose that spike-induced calcium release from IP3Rs leads to SK channel activation, thereby fine tuning membrane excitability in central neurons.

Original languageEnglish
Pages (from-to)2322-2329
Number of pages8
JournalJournal of Neurophysiology
Volume91
Issue number5
DOIs
Publication statusPublished - 01-05-2004

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Pyramidal Cells
Calcium
Apamin
Calcium Signaling
Visual Cortex
Baths
Neurotransmitter Agents
Heparin
Neurons
Membranes

All Science Journal Classification (ASJC) codes

  • Neuroscience(all)
  • Physiology

Cite this

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abstract = "Cooperative regulation of inosiol-1,4,5-trisphosphate receptors (IP 3Rs) by Ca2+ and IP3 has been increasingly recognized, although its functional significance is not clear. The present experiments first confirmed that depolarization-induced Ca2+ influx triggers an outward current in visual cortex pyramidal cells in normal medium, which was mediated by apamin-sensitive, small-conductance Ca 2+-dependent K+ channels (SK channels). With IP 3-mobilizing neurotransmitters bath-applied, a delayed outward current was evoked in addition to the initial outward current and was mediated again by SK channels. Calcium turnover underlying this biphasic SK channel activation was investigated. By voltage-clamp recording, Ca2+ influx through voltage-dependent Ca2+ channels (VDCCs) was shown to be responsible for activating the initial SK current, whereas the IP3R blocker heparin abolished the delayed component. High-speed Ca2+ imaging revealed that a biphasic Ca2+ elevation indeed underlays this dual activation of SK channels. The first Ca2+ elevation originated from VDCCs, whereas the delayed phase was attributed to calcium release from IP3Rs. Such enhanced SK currents, activated dually by incoming and released calcium, were shown to intensify spike-frequency adaptation. We propose that spike-induced calcium release from IP3Rs leads to SK channel activation, thereby fine tuning membrane excitability in central neurons.",
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Small-Conductance Ca2+-Dependent K+ Channels Are the Target of Spike-Induced Ca2+ Release in a Feedback Regulation of Pyramidal Cell Excitability. / Yamada, Shin Ichiro; Takechi, Hajime; Kanchiku, Izumi; Kita, Toru; Kato, Nobuo.

In: Journal of Neurophysiology, Vol. 91, No. 5, 01.05.2004, p. 2322-2329.

Research output: Contribution to journalArticle

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